Distribution of nucleotide event with the permanent adapter. (a) Single-channel recording from the WT-(M113R/N139Q)6(M113R/N139Q/L135C)1-am6amPDP1βCD pore showing dGMP, dTMP, dAMP, and dCMP discrimination with a Gaussian fits. (b) Corresponding residual current histogram of nucleotide binding events, including Gaussian fits. Data acquired under the condition of 400 mM KCl, 25 mM Tris HCl, pH 7.5, at +180 mV in the presence of 10 μM dGMP, 10 μM dTMP, 10 μM dAMP, and 10 μM dCMP, reprinted by permission from Nature Publishing Group: Nature Nanotechnology [59], copyright 2009.
