Preparation of Folate-Conjugated Pluronic F127/Chitosan Core-Shell Nanoparticles Encapsulating Doxorubicin for Breast Cancer Treatment

Table 2

Cellular uptake of DOX by MCF-7 cells treated with free DOX, DOX-loaded chitosan nanoparticles, and DOX-loaded folate-conjugated chitosan nanoparticles for up to 24 hours. Results are presented as percent of the initial concentration of DOX.

Conditions	Cellular DOX uptake (% of its initial concentration)
	1 h	2 h	6 h	12 h	18 h	24 h
Free DOX	26.8±0.58	80±1.3	74.7±2.1	98.0±1.7	93.7±2.7	102.8±4.4
DOX-loaded micelles coated with chitosan	0.5±0.1	0.6±0.2	2.7±0.6	4.5±1.0	11.2±0.8	14.7±1.5
DOX-loaded micelles coated with modified chitosan	0.6±0.4	0.7±0.6	10.9±0.7	12.7±0.7	21.1±1.9	26.3±1.9