Self-Assembling Peptide Nanofiber Scaffold Enhanced with RhoA Inhibitor CT04 Improves Axonal Regrowth in the Transected Spinal Cord
Figure 1
The implants of various SAPNS reconstructed the injured spinal cord. (a–d) Macroscopic observations of lesion sites immediately after surgery. (a) Sham surgery; (b) saline group; (c) SAPNS with vehicle group; (d) SAPNS+CT04 combination group. (e) HE staining shows the lesion area was reconstructed 12 weeks after SAPNS transplanted. (f) HE staining shows various cells in the grafted scaffold, which include fibroblast (black arrow), macrophage (yellow arrow), and lymphocyte (arrow head). (g–i) Immunostaining shows the GFAP-positive astrocytes (g), IBA1 positive microglias (h) and ED1 positive macrophages (i) in the grafted scaffold. (j) shows the Dextran, a red fluorescent dye, was partially diffused from the mixture of SAPNS and Dextran at 7 days after transplantation. Scale bar is 1000 μm for (a–e) and (j), 50 μm for (f-g).
