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Journal of Nanomaterials
Volume 2012, Article ID 730746, 12 pages
Research Article

Green Synthesis of Robust, Biocompatible Silver Nanoparticles Using Garlic Extract

1Department of Chemical and Biomolecular Engineering, Clemson University, Clemson, SC 29634, USA
2Department of Bioengineering, Clemson University, Clemson, SC 29634, USA

Received 22 January 2012; Accepted 16 February 2012

Academic Editor: Chunyi Zhi

Copyright © 2012 Gregory Von White et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


This paper details a facile approach for the synthesis of stable and monodisperse silver nanoparticles performed at ambient/low temperature, where Allium sativum (garlic) extract functions as the silver salt reducing agent during nanoparticle synthesis as well as the postsynthesis stabilizing ligands. Varying the synthesis conditions provides control of particle size, size-distribution, and kinetics of particle formation. Infrared spectroscopy, energy dispersive X-ray chemical analysis, and high-performance liquid chromatography indicated that allicin and other carbohydrates in the garlic extract are the primary nanoparticle stabilizing moieties. The synthesized silver nanoparticles also demonstrate potential for biomedical applications, owing to (1) enhanced stability in biological media, (2) resistance to oxidation by the addition of H2O2, (3) ease and scalability of synthesis, and (4) lack of harsh chemicals required for synthesis. Cytotoxicity assays indicated no decrease in cellular proliferation for vascular smooth muscle cells and 3T3 fibroblasts at a concentration of 25 μg/mL, confirming that silver nanoparticles synthesized with garlic extract are potential candidates for future experimentation and implementation in the biomedical field.