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Journal of Nanomaterials
Volume 2012, Article ID 734398, 9 pages
Research Article

In Vitro Cytotoxicity Assay on Gold Nanoparticles with Different Stabilizing Agents

1Department of Physics, Sri Ramakrishna Institute of Technology, Pachapalayam, Perurchettipalayam, Coimbatore 641010, India
2Department of Physics, Government College of Technology, Thadagam Road, Coimbatore 641013, India

Received 9 April 2012; Revised 16 July 2012; Accepted 20 July 2012

Academic Editor: Xiaoming Li

Copyright © 2012 S. Vijayakumar and S. Ganesan. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Noble gold nanoparticles (AuNps) are generally nontoxic due to their inert nature. The gold nanoparticles are easily tagged with various proteins and biomolecules rich in aminoacid leading to important biomedical applications including targeted drug delivery, cellular imaging, and biosensing. In this study, three cytotoxicity detection assays 3-(4,5-dimethylthiozol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT), neutral red cell, and lactate dehydrogenase (LDH) on gold nanoparticles stabilized with citrate, starch, and gum arabic are used. The assays used are based on different mode of detection like LDH release, MTT metabolism, and neutral red uptake. We found that the AuNps stabilized with citrate are very sensitive to the change of concentration and time assay compared to starch and gum arabic stabilized gold nanoparticles.