Advanced Nanohybrid Materials: Surface Modification and ApplicationsView this Special Issue
Research Article | Open Access
Synthesis of a Layered Organic-Inorganic Nanohybrid of 4-Chlorophenoxyacetate-zinc-Layered Hydroxide with Sustained Release Properties
A zinc-layered hydroxide-4-chlorophenoxy acetate (4CPA) organic-inorganic nanohybrid was prepared using a simple direct reaction of 4CPA anions with ZnO under an aqueous environment to be used as a controlled release formulation of the herbicide, 4CPA. The concentration of the active agent, 4CPA, was found to be a controlling factor for the formation of a pure phase well-ordered nanolayered hybrid in which it could be synthesised at 0.2 M 4CPA. ZnO shows a well-defined grain structure of variable size in the nanometre range. However, the formation of the 4CPA-ZLH nanohybrid resulted in a flake-like fibrous structure. On heating at 500°C for 5 h under atmospheric conditions, the nanohybrid transformed back to a well-defined grain structure, as previously observed with the starting material, ZnO. The release of 4CPA was found to occur in a controlled manner and was generally governed by pseudo-second-order kinetics.
Layered metal hydroxide compounds with inorganic brucite-like structures can be generally classified into two major types, layered double hydroxides (LDHs) and layered metal hydroxides (LMHs). They can be represented by the general formula, and , respectively, where is a divalent cation (, , , , , , ), is a trivalent cation (, , , , , ) and is an interlayer anion (, , , , , and many other inorganic anions). For LDH, the value is the charge density, that is, the molar ratio of [1, 2]. LMH consists of two parts: positively charged brucite-like inorganic layers and exchangeable anions and water molecules in the interlayer. These properties give LMH wide applications in industrial and environmental research from catalysts  to antacids  and from magnetic materials [5, 6] to controlled release formulations of various active agents such as DNA , drugs [8, 9], and agrochemicals [10–15].
One LMH which has been the subject of intense research lately is zinc-layered hydroxide (ZLH). This is due the fact that ZLH has very similar properties to those of LDH. The 2D layered inorganic material is composed of layers with octahedral coordinated zinc cations, in which 1/4 of them are displaced out of the layer, leaving an empty octahedral site which forms cationic centres tetrahedrically coordinated to the top and bottom of the octahedral sheet. Water molecules occupy the apices, and nitrate counter ions are free between the interlayers .
Controlled release formulations (CRF) have been used as an approach to exploit the most effective use of herbicides and at the same time to prevent environmental pollution . Ideally, the CRF should be able to release herbicides at a level required to manage the crops, therefore preventing problems such as leaching, evaporation, degradation, and excessive use of harmful chemicals . Various matrices have been proposed for use as pesticide CRF. The active agent should be immobilised or intercalated into an inert matrix and gradually released over time . The use of natural and synthetic clay-type materials in the preparation of CRF is of current interest due to their tailor-made properties and relatively low cost of preparation.
In this study, we discuss our work on the intercalation of 4CPA, an herbicide, into the interlayer of ZLH by a simple synthesis method, that is, by the direct reaction of ZnO with 4CPA under an aqueous environment for the formation of a new controlled release formulation of the organic-inorganic-layered nanohybrid type. In addition, the controlled release property of the resulting nanohybrid will be also discussed.
2. Materials and Methods
All chemicals used in this synthesis were obtained from various chemical suppliers and used without further purification. All solutions were prepared using deionised water with resistivity of 18.2 M-cm.
In this study, 0.05 g ZnO (Acros), was discharged into 100 mL deionised water. Solutions of 4CPA (Aldrich) with concentrations of 0.025–0.1 M were added to the ZnO solutions and aged at 70°C in an oil bath shaker for 18 hours. The synthesised material was centrifuged, thoroughly washed with deionised water and dried in an oven at 70°C. The resulting material was then powdered and stored in a sample bottle for further use and characterisation.
Powder X-ray diffraction patterns were recorded at 2–60° on a Shimadzu diffractometer XRD-6000 using radiation at 40 kV and 30 mA. FTIR spectra of the materials were recorded over the range 400–4000 cm on a Perkin-Elmer 1752X spectrophotometer using the KBr disc method. A CHNS analyser, model CHNS-932 of LECO Instruments was used for CHNS analyses. Thermogravimetric and differential thermogravimetric analyses (TGA/DTG) were carried out using a Mettler Toledo instrument. Surface characterisation of the material was carried out using a nitrogen gas adsorption-desorption technique at 77 K using a Micromeritics ASAP 2000. The surface morphology of the samples was observed by a field emission scanning electron microscope (FESEM), using a JOEL JSM-6400. UV-vis spectra were obtained using a Perkin Elmer UV-Visible Spectrophotometer, Lambda 35. Surface analysis of the nanohybrid in powder form was studied using an X-ray Photoelectron Spectrometer (XPS), Kratos Ultra. Samples were analysed at torr using monochromatic as the X-ray source and data were collected based on wide and narrow scans of the elements of interest.
3. Results and Discussion
3.1. Powder X-Ray Diffraction
The powder X-ray diffraction (PXRD) patterns of ZnO, 4CPA, and the nanohybrids prepared using 0.05, 0.10, and 0.20 M 4CPA are shown in Figure 1. The PXRD patterns of ZnO show that the sample was of a pure phase with good crystallinity as shown by the presence of typical ZnO peak patterns (Table 1).
|(b) XPS analysis (value in Ev)|
|(c) Thermal property|
|(d) aKinetic equations|
| is the percentage release of 4CPA at time, (min), and is a constant. , , and are the rate constant, the initial, and final concentration of 4CPA, respectively.|
The resulting materials obtained by direct reaction between ZnO and 4CPA (0.05–0.20 M) showed well-ordered 2D-layered nanohybrids, labelled as ZCPAs. The average basal spacing of 21.26 and 21.14 Å was observed when 0.05 and 0.10 M 4CPA was used, respectively, in the synthesis, compared to 19.03 Å when 0.20 M 4CPA was used (see Figure 1) with 4, 3, and 5 harmonics, respectively. This confirmed the formation of layered nanohybrids in which ZnO under an aqueous environment was transformed into a metal layered hydroxide, and at the same time, the intercalation of the 4CPA anion into the interlayer of the zinc-layered hydroxide took place.
It is believed that a “dissociation-deposition” mechanism governs the formation of the nanohybrid synthesised by a direct reaction between 4CPA and ZnO under an aqueous environment. ZnO was first hydrolysed followed by the formation of Zn(OH)2 on the surface of solid particles. The formation of Zn(OH)2 then subsequently formed thin layers on the oxide particle surfaces. The dissociation of Zn(OH)2 occurred at the solution-solid interface and formed Zn2+ species. This species was then reacted with the hydroxyl group of 4CPA anions and water in solution, to generate the layered ZCPA nanohybrid. As a result, a nanolayered hybrid in which 4CPA was intercalated into the interlayer of zinc-layered hydroxides was formed . Also, shown in Figure 1, ZCPA nanohybrids synthesised using 0.10 and 0.20 M 4CPA showed a relatively purer phase than the one synthesised at 0.05 M. The latter showed the presence of ZnO, indicated by an incomplete reaction which resulted in the remaining ZnO phase. In contrast, no ZnO phase could be observed for the nanohybrids prepared at 0.10 M and 0.20 M 4CPA, indicating that a complete reaction between the 4CPA anion and ZnO had taken place. As a result, the formation of phase pure zinc-layered hydroxide nanohybrid in which the 4CPA anion was completely intercalated into the inorganic interlayer host could be observed. The PXRD pattern of ZCPA synthesised at 0.20 M 4CPA showed a well-ordered nanolayered hybrid with up to five harmonics, from which the average basal spacing of 19.03 Å of the material was deduced, showing a long-range order of the layer packing. The basal spacing was very similar to that of the 4CPA-Zn/Al-LDH nanohybrid, which is 20.1 Å .
3.2. Fourier Transform Infrared
Figure 2 shows the FTIR spectra of ZnO, 4CPA, and ZCPA. The FTIR spectrum of pure ZnO showed a band at 382 cm, which was due to zinc-oxygen sublattices . For 4CPA, a broad absorption band at 3102 cm was due to O–H stretching vibration of the hydrogen-bonded and nonhydrogen-bonded hydroxyl groups from the hydroxide layers and interlayer water in 4CPA. The band at 2911 cm was due to the presence of C–H stretching vibrations of aromatic compounds. In addition, H–O–H bending vibration in 4CPA could be observed at 1873 cm and a sharp, strong, and distinct band at 1705 cm indicated the C=O functional group. Both bands at 1487 cm and 1426 cm were attributed to the C=C stretching vibration of the aromatic ring. The C–O–C stretching vibration was observed at 1230 cm and a band at 703 cm was due to the C–Cl stretching vibration.
As shown in the figure, the nanohybrid ZCPA showed a broad absorption band at 3458 cm which corresponded to the O–H stretching vibration due to adsorbed and/or interlayer water. The band at 2970 cm was due to the C–H stretching vibration of the aromatic group while the H–O–H bending vibration for ZCPA was observed at 1888 cm. A band at 1547 cm was due to the stretching vibration of aromatic compounds. Bands at 1487 cm and 1409 cm were attributed to the C=C stretching vibration of the aromatic ring. Furthermore, the C–O–C stretching vibration was observed at 1220 cm and a band at 713 cm corresponded to the C–Cl stretching band.
The FTIR spectrum of the ZCPA nanohybrid generally showed characteristic bands, indicating the contribution of 4CPA and ZnO. These data demonstrate that the 4CPA anion had been intercalated into the interlayer of ZLH. However, some of the bands shown were slightly shifted in position, which indicated the existence of interactions between the 4CPA anionic guest and the ZLH host as a result of the formation of new bonds due to the intercalation process.
3.3. Organic Content, XPS, MS, and Thermal Studies
The organic loading of the ZCPA nanohybrid gave an indication of the degree of intercalation of 4CPA, C8H6ClO3. If only adsorption of 4CPA occurred, then the XRD pattern of the resulting material should have been composed of the component phases separately, ZnO and 4CPA. The carbon and hydrogen contents of 4CPA and ZCPA nanohybrid obtained by CHNS analysis are shown in Table 1. The results show that 4CPA contained 51.2% carbon compared to 21.9% for the nanohybrid, ZCPA. Therefore, the loading percentage of 4CPA in the nanohybrid of ZCPA was estimated to be 45.4% (w/w). This result also confirmed the intercalation episode of the 4CPA anion into the inorganic interlayer of ZLH. Otherwise, adsorption of 4CPA occurred, resulting in the observation of the 4CPA phase in the resulting XRD patterns.
In order to confirm that the organic moiety that was actually intercalated into the ZLH interlamellae was 4CPA, the direct injection mass spectra (DIMS) were studied. The DIMS of 4CPA (Figure 3) showed major peaks at values of 186, 141, 128, 111, 75, 50, and 28 and similar peaks could be also observed for the nanohybrid, ZCPA. The molecular ion at 186 and 267 corresponded to the molecular formula of 4CPA (C8H7ClO3) and C8H6ClO3-ZnO, respectively. The latter particularly showed the formation of a bond between 4CPA and Zn–O in the ZLH. Again, this demonstrated that the intercalation of 4CPA into ZLH had been successfully accomplished and confirmed the formation of the ZCPA nanohybrid in which the 4CPA organic moiety was bound with the inorganic ZLH cation, Zn, in the interlamellae of ZLH.
XPS spectra of ZCPA nanohybrid are given in Figure 4 and the analysis is presented in Table 1. Generally, expected elements such as C, O, Zn, and Cl were detected from a wide scan of the photoelectron peaks of the , and spectra of the sample. The related binding energies were compared with references for their elemental chemical states . Carbon () was observed mainly as a terminal hydrocarbon ( = 284.5 eV) and C–Cl from 4CPA. Oxygen (), observed at 530.3 and 531.7 eV was due to Zn–O and –OH, respectively. There was only one chemical state of Zn () at 1021.7 eV that could be observed in the XPS spectrum, which could be attributed to the Zn of Zn–O–H. Chlorine () in the form of a single organo-chlorine was observed at a binding energy of 200.26 eV, which was expected from the 4CPA compound. In general, all the expected elements and their chemical states were in agreement with the assumption that organocompounds were bonded to Zn. This shows that the organic moiety, 4CPA was directly bonded to Zn, and that it was intercalated between ZLH interlayers in agreement with the DIMS study.
Thermogravimetric analysis of 4CPA showed a strong, intense, and sharp peak at a temperature maximum of 243.5°C with weight loss of 97.5% (Table 1). This corresponded to complete decomposition of the organic compound, 4CPA. For ZCPA, there were four intense and sharp peaks that could be seen clearly. The first temperature maximum was at 92.6°C with a weight loss of 6.8%. This was due to the removal of surface physisorbed water molecules. The weight loss of 6.2% at 155.0°C was attributed to the removal of interlayer anions and dehydroxylation of the hydroxyl layer. About 30.1% weight loss at 294.4°C was due to the decomposition of the intercalated organic moiety, the 4CPA anion, between the inorganic-ZLH layers (Figure 5).
The thermal study showed that 4CPA intercalated into the inorganic host, ZLH was thermally more stable than its counterpart in the nonintercalated form. TGA/DTG thermograms of ZnO showed that it was a very stable compound thermally with weight loss of 0.9% at 100.6°C, attributed to surface-adsorbed water molecule from the atmosphere.
3.4. Surface Morphology and Surface Area
Field emission scanning electron microscope (FESEM) was used to study the surface morphology of the materials. The surface morphology of ZnO showed well-defined hexagonal and square-like structures with various shapes and sizes at the nanometre scale. This structure was transformed into a thin, sheet-like structure when the ZCPA nanohybrid was formed by a direct reaction of ZnO with 4CPA under an aqueous environment, as shown in Figure 6. This showed that the intercalation of 4CPA into the interlayer of zinc-layered hydroxide resulted in a change of the surface morphology from a well-defined fine hexagonal- and square-like structure to a thin, sheet-like structure with a thickness in the nanometre range. This is slightly different compared to the rosette-like structure that has been observed for the [4-(2,4-dichlorophenoxybutyrate)-zinc-layered hydroxide] nanohybrid .
When the ZCPA nanohybrid was calcined at 500°C for 5 h, the structure of thin, sheet-like nanostructures was transformed back to the original ZnO structure (Figure 6(c)) reverting back to the irregular hexagonal- and square-like structure. This indicated that the organic moiety, 4CPA, intercalated into the interlayer of inorganic ZLH and was completely decomposed by heating at 500°C. At the same time, the Zn component was oxidised, resulting in the formation of ZnO.
The BET surface area was measured for ZnO and ZCPA by the gas adsorption technique and the surface areas were determined to be 4 and 6 m2/g, respectively, (Table 1). Relative to ZnO, the intercalation resulted in an increase of the BET-specific surface area by about 50%. This is in agreement with the formation of mesopores (Type IV isotherm) created by the formation of a layered ZCPA nanohybrid.
3.5. Controlled Release and Kinetics
Figure 7 shows the release profiles of 4CPA from the interlamellae of the ZCPA nanohybrid into 0.001 and 0.002 M Na2CO3 aqueous solutions. As shown in the figure, the accumulated 4CPA released into the aqueous solution increased with contact time when ZCPA was placed in the aqueous solutions. For the 0.002 M Na2CO3 solution, the release rate was found to be rapid for the first 1400 minutes, followed by a sustained release thereafter. Equilibrium was achieved at around 1685 minutes with 65% of the 4CPA released from the interlayer of ZCPA. On the other hand, for the 0.001 M Na2CO3 aqueous solution, the amount of 4CPA released was found to be about 12% with a rapid release rate for the first 1450 minutes, followed by a slow release thereafter. Equilibrium was achieved at 1720 minutes. This result indicates that the higher the concentration of aqueous solution used, the higher the percentage release of 4CPA from the interlamellae of ZCPA nanohybrid. This was due to the higher concentration of carbonate from the aqueous solution to be ion-exchanged with 4CPA from the interlamellae of ZCPA nanohybrid material.
The release kinetics of 4CPA from the interlamellae of ZLH were evaluated using various kinetics models, namely, zeroth-, first-, pseudo-second order, as well as parabolic diffusion equations (Table 1).
An attempt was made to fit the data for 4CPA release into these equations, and the obtained results are shown in Figure 8. Linear regression () showed that a pseudo-second-order kinetic model fit better than the other models used in this work with an value of 0.927 and 0.851 for 0.001 M and 0.002 M sodium carbonate, respectively. This shows that the release of the organic moiety from the inorganic ZLH interlamellae involved the dissolution of the nanohybrid as well as ion exchange between the intercalated anions in the interlamellae host and the carbonate anions in the aqueous solution which was controlled by pseudo-second-order kinetics. These results are very similar to the results of a previous study .
An organic-inorganic nanohybrid of zinc-layered hydroxide-4-chlorophenoxyacetate can be prepared using a simple direct reaction of the 4CPA anion with ZnO under an aqueous environment. The concentration of 4CPA was found to be a controlling factor in determining the formation of a pure phase, well-ordered nanolayered hybrid material. The nanohybrid showed a controlled release property, and the release was governed by pseudo-second-order kinetics. Therefore, such a material could be used as a controlled release formulation of herbicides.
Funding for this research was provided by the Ministry of Higher Education of Malaysia (MOHE) under the FRGS Grant no. 02-11-08-615FR. N. F. Nazarudin thanks Jabatan Perkhidmatan Awam Malaysia for the scholarship.
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