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Journal of Nanomaterials
Volume 2012, Article ID 865186, 7 pages
http://dx.doi.org/10.1155/2012/865186
Research Article

Synthesis of Antibodies-Conjugated Fluorescent Dye-Doped Silica Nanoparticles for a Rapid Single Step Detection of Campylobacter jejuni in Live Poultry

1Risk and Decision Analysis Laboratory (RADAL), Food Safety Center, KMUTT and Department of Microbiology, Faculty of Science, King Mongkut’s University of Technology Thonburi, Bangkok 10140, Thailand
2Department of Microbiology, Faculty of Science, Kasetsart University, Bangkok 10900, Thailand
3Department of Physics, Faculty of Science, King Mongkut’s University of Technology, Thonburi, Bangkok 10140, Thailand
4Department of Science Services, Ministry of Science and Technology, Bangkok 10400, Thailand

Received 28 September 2011; Revised 28 December 2011; Accepted 29 January 2012

Academic Editor: Claude Estournes

Copyright © 2012 Wachira Tansub et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

The preparation of antibodies-conjugated fluorescent dye-doped silica nanoparticles (FDS-NPs) was developed to detect Campylobacter jejuni cells under a fluorescence microscope. The particles prepared by sol-gel microemulsion techniques have a round shape with an average size of 43 ± 4 nm. They were highly photo stable and could emit strong orange fluorescent for 60 min. Both amine- and carboxyl-functionalized properties were evident from FTIR and FT Raman spectra. The FDS-NPs conjugated with antibodies against C. jejuni were well dispersed in PBS solution at 20 mM of NaCl. The conjugation with monoclonal antibodies against C. jejuni was successful. The direct observation of the antibodies-conjugated FDS-NPs- that bounds C. jejuni with Petroff Hausser counting chamber at 40x was clear. The different focus lengths clearly separated bound and unbound FDS-NPs under the microscope. We successfully synthesis the bio-conjugated dye doped silica nanoparticles for C. jejuni that are easy to use and giving clear detection in due time.