Figure 5: Nuclear translocation of β-catenin on ripples is sensitive to Gd3+. Confocal microscopy images of β-catenin distribution (red) in HMEC grown on the indicated substrate. Cells were incubated with 10 μM Gd3+ for 6 h at 37°C (a). Lower panels: representative profile plots along the indicated cell section (yellow line) show intensity distribution of β-catenin as relative immunofluorescence intensity ( -axis) along the distance in pixel ( -axis). Scale bars represent 10 μm. Bar graphs representing the nuclear to cytosolic ratio of β-catenin immunofluorescence (b); values ± SEM; from 3 independent experiments, calculated from 3 different images of each experiment; ; compared to CTRL.