β-Tricalcium Phosphate Micron Particles Enhance Calcification of Human Mesenchymal Stem Cells In Vitro

Figure 2

Characteristics of synovial MSCs. (a) Colony forming potential. (b) Chondrogenesis. (c) Adipogenesis. (d) Calcification. (e) Osteogenesis-related gene expressions by real-time PCR analysis. Averaged relative mRNA expression level with standard deviation is shown (𝑛=6, *𝑃<0.05, **𝑃<0.01 by the paired t-test). Synovial MSCs cultured in calcification medium were compared with undifferentiated MSCs. (f) Averaged alkaline phosphatase activity with standard deviation (𝑛=7, **𝑃<0.01 by the paired t-test). (g) Epitope profile of synovial MSCs. Positive expression rates (%) with standard deviation are shown (𝑛=4).

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