β-Tricalcium Phosphate Micron Particles Enhance Calcification of Human Mesenchymal Stem Cells In Vitro
Figure 4
Effect of -TCP micron particles on calcification of MSCs. (a) Alizarin red staining. MSCs were cultured without or with -TCP micron particles in the contact or the noncontact condition for 21 days and in the contact condition for 3 days. As a positive control, MSCs were cultured in calcification medium. (b) High magnified pictures for MSCs stained with alizarin red. Black arrow indicates -TCP micron particles stained with alizarin red located outside of MSCs. (c) Stained area at 21 days with alizarin red, which was calculated by subtracting stained area at 3 days in the contact condition. Averaged area with standard deviation is shown (, * by the Kruskal-Wallis test). (d) Osteogenesis-related gene expressions by real-time PCR analysis. Averaged relative mRNA expression level with standard deviation is shown (, * by the Kruskal-Wallis test).
