Research Article | Open Access
Marcio N. Gomes, Jackeline B. Brito, Josmary R. Silva, Nara C. de Souza, "Layer-by-Layer Films from Wine: An Investigation of an Exponential Growth Process", Journal of Nanomaterials, vol. 2013, Article ID 437203, 7 pages, 2013. https://doi.org/10.1155/2013/437203
Layer-by-Layer Films from Wine: An Investigation of an Exponential Growth Process
We report on the preparation and study of layer-by-layer films of wine alternated with bovine serum albumin (BSA). We found that the exponential and/or linear growth of the films is dependent on the deposition time. Atomic force microscopy images were analysed using scale laws and the fractal dimension, and the results suggested that the BSA/wine film growth regime is determined by sub-bilayer or bilayer growth. Exponential growth was associated with a sub-bilayer deposition regime, whereas linear growth was associated with a bilayer deposition in which a constant amount of material is deposited.
Grapes and wine have many constituents, such as proteins, sugars, and vitamins, that have nutritional value and thus improve quality of life [1, 2]. Extensive research has examined the beneficial effects of moderate wine intake, which provides antioxidant effects, decreases levels of low density lipoprotein (LDL), and prevents diabetes, cardiovascular diseases, and neurodegenerative diseases [3, 4]. Although grapes and wine have similar phytotherapic effects, the literature indicates that wine has enhanced therapeutic properties due to the fermentation process, likely as a result of alcohol production, which improves the vasodilator properties of wine .
Among the molecules identified in wine, resveratrol and quercetin (flavonol) are widely investigated because they have anti-inflammatory, antifungal, cardioprotective [5, 6], and antitumor properties . Previous research [8–12] has studied the biomedical characteristics of these two molecules in order to correlate their properties with the beneficial effects of moderate wine intake [3, 5].
Because of the complexity of the various compounds identified in wine, most studies have investigated isolated molecular groups in an aqueous or alcoholic solution [8, 10]. Most studies investigating wine have characterised the processes of winemaking and its chemical composition [13–17]. More recent work has investigated the interaction of wine with various ligands [3, 18, 19]; however, the mechanisms responsible for these interactions are not further explained.
Films obtained from the layer-by-layer technique (LbL) enable the immobilisation of different molecules on a single substrate and allow the characterisation of a complex multifunctional surface . Two kinds of growth regimes during the build-up of LbL films have been reported: linear growth and exponential growth. For many years, linear growth was the only regime reported [20, 21] for the LbL technique. However, in 1999, Elbert et al. reported a ground-breaking discovery in the LbL technique, observing an exponential growth of LbL films from poly(L-lysine) (PLL) alternated with sodium alginate (Alg) . To explain the exponential growth, the authors hypothesised that the increase in the amount of deposited material in each layer was caused by an increase in the film roughness, which was associated with the available deposition area and a fixed number of deposition sites for each area . Since the report of Elbert et al., several systems have been shown to exhibit exponential growth, that is, PLL/hyaluronan [23, 24], PLL/poly(L-glutamic acid) , Chitosan/HA [26, 27], and PAH/poly(L-glutamic acid) . Interestingly, a regime transition from linear to exponential has been noted to change the ionic strength, polydispersivity, and temperature of the films .
Despite the numerous investigations to date, all previous studies on wine have examined fabricated wine (in the liquid phase), and to the best our knowledge, there are no studies on LbL films from wine. The advantage of using the film form of wine components is that several solid state experimental techniques can be used for characterisation. In addition, LbL films based on wine and BSA protein emerge as an experimental model for studies on the interaction between wine components and protein. Polyphenols in wine are capable of forming stable combinations with proteins. Although various types of interactions have been observed, their mechanisms have not been completely explained . The two main types are hydrophobic interactions  and hydrogen bonds , while ionic or covalent bonds are probably present but less important. In this paper, we report the successful preparation of LbL films from wine alternated with BSA and an investigation of the growth regimes of the BSA/wine LbL film. To elucidate the exponential growth, we have used scale laws and fractal concepts to analyse the AFM results of the films.
2. Materials and Methods
Portuguese red wine made from Vitis vinifera L. cv. Touriga Nacional (2007 vintage) was obtained from commercial wineries. Wine was used as received without any dissolution immediately after the bottle was opened. BSA (fraction V, purity 96–100%) from Acros Organics was used as received. The experimental film fabrication procedures are essentially the same as those described by Decher . Since our experience shows that the rinsing step after the deposition of BSA layer leads to the formation of surface with a low coverage ratio, the washing step was excluded. To build the multilayers of BSA alternated with wine (hereinafter BSA/wine films), wine (pH 3.8) was alternated with 0.5 g/L BSA diluted in ultrapure water. The pH of the BSA solution was adjusted to 7.5 (close to the physiological pH) by adding appropriate amounts of ammonium hydroxide. The films were adsorbed on quartz (). The absorbance of the wine, which is proportionate to the adsorbed amount of the wine solute, was monitored by measuring the UV-Vis absorption spectra with a double-beam Thermo Scientific spectrophotometer, model Genesys 10. The multilayer films were assembled by alternating the immersion of the substrate into the BSA and into the wine solution. After each deposition step, the substrates were dried under an air flow. The morphology of the films was studied with a NanoSurf Instruments atomic force microscope EasyScan II in the tapping mode ( pixels) under ambient conditions. The tapping mode was selected instead of the contact mode because it is less damaging to the surfaces under investigation , as the transferred energy is less and the tip does not exert a significant lateral force on the surface. A sample area of was scanned, and an image was acquired. The films roughness was determined using NanoSurf Instruments software.
3. Results and Discussion
The inset in Figure 1 shows the spectra of a BSA/wine LbL film with bands at 280 and 530 nm. In wines, anthocyanins absorb between 520 and 530 nm [35, 36], and the BSA has a band at 280 nm due to the amino acids tyrosine and tryptophan . The adsorption process was monitored via UV-Vis spectroscopy after the deposition of each bilayer onto the quartz substrate using an absorption wavelength of 530 nm. The curve in Figure 1 was obtained after the deposition of BSA using an immersion time of 10 minutes for all bilayers. The immersion time of the films in the wine solution was varied from 5 s to 120 min, and an increase in the absorbance at 530 nm was observed (Figure 1).
Figure 1 shows the initial formation of a plateau at about 1.5 h. The formation of the plateau results from saturation sites, indicating formation of a complete layer. Thus, we can infer that the BSA layer will be completely filled after 2 h of wine immersion. In this case, we can expect the growth process of the BSA/wine film (10 min/2 h) to be linear, with approximately the same amount of material being deposited in each bilayer. Further, we can expect that any other deposition time (shorter times) will lead to a less effective growth with incomplete layers.
3.1. Growth Regime of the BSA/Wine Films
An important characteristic to be studied in the growth of LbL films is the adsorbed amount at each deposition step. Figure 2 depicts the absorbance as a function of the bilayer number for the BSA/wine LbL films at different times during the wine deposition.
Based on the results shown in Figure 1, the multilayers films were assembled by alternating the immersion of the substrate into the BSA for 10 minutes and into the wine solution for 5 min, 30 min, and 2 h. After each deposition, the substrates were dried under an air flow. As shown in the inset (Figure 2), the adsorbed amount of mass, which is proportional to absorbance [21, 29], increases linearly with an increase in the number of bilayers when the deposition time in the wine is 2 h, as expected. On the other hand, when the immersion time in the wine solution is 5 min, the growth is exponential, and surprisingly, we found that the transition from exponential to linear growth occurs with an immersion time of 30 minutes in the wine solution. Unlike the linear growth, where the results suggest that the same amount of material was adsorbed in each deposition step, the exponential growth indicates that in each deposition cycle the adsorbed amount of material increases. This behaviour was identified in several systems and was associated with in- and out-diffusion processes [23–25, 38–41]. Recently, some authors have proposed alternative explanations for the exponential growth, which are based on surface structure models, such as island and dendritic models . However, the mechanisms that give rise to the exponential growth were not well elucidated.
3.2. Film Growth Analysis Using Dynamic Scale Laws and Fractal Concepts
To explain the exponential and linear growth behaviour of the BSA/wine (10/30 min) films, we performed an analysis of the AFM images of these film surfaces using fractal concepts and scale laws . Figure 3 shows the AFM images of the BSA/wine films for 1, 3, and 7 bilayers in which the top layer is wine.
The images were taken in a scan window of . To characterise the interface quantitatively, we determined the roughness exponents, , using scale laws . Figure 4 shows the log versus log plot, where is the root-mean-square (RMS) roughness and is the scan window size. In the linear region, where the scaling law is satisfied, the value can be obtained from the slope of the curve, and the fractal dimension of a surface can be calculated using .
Table 1 shows the roughness RMS along with the roughness exponent and the fractal dimension for the LbL films with 1, 3, and 7 bilayers. The roughness of the interface is defined by the RMS fluctuation in the height between the structures forming on the surface. The fractality relates to the effect of changes in the scale of observation on the roughness.
As observed in Figure 3, small aggregates [21, 43–46] can be noted for the 1-bilayer films with whole substrate coverage. For the 3-bilayer films, there are large and small aggregates emerging from the surfaces of the films. For the 7-bilayer films, the surface is smoother compared with the 1 and 3 bilayer films. From the viewpoint of the fractal dimension, for the 1-bilayer film, the small aggregates shown in Figure 3 are associated with the fractal dimension of . For the 3-bilayer film, the small aggregates (revealed on the top of the larger aggregates) contribute to an increase in the fractal dimension () as compared to the 1-bilayer film. Finally, the smooth surface noted in the 7-bilayer films is related to a lower fractal dimension () among the films as a result of the coalescence of large islands, which can arise from a process of aggregation.
As shown in Figure 2, the BSA/wine (10/30 min) film has two growth regimes, exponential and linear, that can be explained in the following way: the exponential regime is associated with sub-bilayer growth, which results from the development and coalescence of islands (Figure 5(b)). These islands are aggregates that can arise from the interplay between the deposition, diffusion, aggregation, and desorption processes evolving with time until a full bilayer is formed. The linear regime is associated with bilayer deposition (with the same amount of mass deposited in each layer) of BSA/wine, as it is the case with an immersion time in wine of 2 h. The formation of island on the films is likely due to an inhomogeneous distribution of the charge density on the surface of the BSA monolayer. Sites with a higher charge density should attract more molecules and smaller aggregates than sites with a lower charge density. Our hypothesis of the sub-bilayer and bilayer growth regimes is supported by the findings of Picart et al.  and Lavalle et al. , who showed that films with islands display exponential growth.
According to the model of the nucleation and growth of islands, the deposited grains begin to coalesce, thus achieving the growth process of the film . At this stage, the grains begin to fill the voids created by the deposition as described by the Kardar-Parisi-Zhang (KPZ) model, which predicts lateral growth  and is a model well established for studying interface growth. KPZ is one of the models used to describe stochastic formation at an interface, which can be related to the growth of a film surface [47–49]. In this model, it is assumed that the interface grows as a result of the deposition of particles and the surface relaxation produced by lateral growth and desorption. With the voids being filled, the surfaces of the grains become larger and less fractal, and this process contributes to reducing the fractality of the system. The exponent in the KPZ model is close to 0.4 [34, 50, 51], and this value is in good agreement with the values found for the 1- and 3-bilayer films.
For our films, the fractal dimension is ca. 2.2 for the 7-bilayer films. With an increasing number of bilayers, there is a higher probability that the molecules will adsorb onto the empty sites formed at the top of the islands structures , as shown in the morphology for the LbL films with 1 and 3 bilayers (Figure 3), and thus, the structures are smooth. The fractal dimension is close to 2, as , and indicates an Euclidean surface . In fact, for the 7-bilayer film, is comparable to the values derived from the growth models incorporating surface diffusion , which is predicted by the stochastic differential equation of the variation in the height () of the interface as a function of time [43, 53]: The first term on the right-hand side of the equation represents the surface relaxation, and represents the random fluctuations that occur during the deposition. The origin of is the surface diffusion. This equation provides a description of the morphology and dynamics of the interface at a large length scale and for long times (after several bilayers).
In summary, we propose to correlate the exponential growth with the fractality of the surfaces. In Figure 2 (BSA/wine—10/30 min), the curve indicates that for the first bilayers, the quantity of the material increases slowly. This stage may be associated with the nucleation process, which corresponds to the deposition of small grains or islands on the substrate. These small islands appear with dimensions of approximately 100 nm, as observed in the height profile (Figure 5(a)), and generate a surface with a high fractality (). Surfaces with a high fractality may be related to the increase in area available for deposition. The surface analysed for the LbL film with 3 bilayers shows that the fractality further increases from 2.55 to 2.69, indicating that the area available for deposition continues to increase, which may explain the exponential growth (Figure 2). When the islands coalesce, the deposition rate decreases, and the exponential growth transitions to linear growth .
Layer-by-layer films of wine alternated with bovine serum albumin (BSA) were obtained for the first time, demonstrating the effectiveness of the LbL technique. The regime of film growth depends on the time of the deposition in the wine solution. This time can be easily determined through experiments on the kinetics of the bilayers. Times shorter than necessary to completely fill the layer lead to the onset of an exponential growth regime that was characterised by the deposition of islands that are responsible for the formation of incomplete bilayer (sub-bilayers). These islands contribute to the increased fractality of the surface, which is directly related to an increase in the deposition area available. With the growth and coalescence of islands, a full bilayer is formed (linear growth).
By using scale laws and the fractal dimension, we have characterised the films and found for 7-bilayer films, which is close to the value predicted by the stochastic differential equation (). The growth for the 1- and 3-bilayer films was explained by the KPZ model, which helps in extracting general features of the system under study. Our results indicate that the use of an interface growth analysis employing scale laws and fractal concepts as well as stochastic growth equations is an effective approach for investigating LbL film growth.
Finally, the analysis in this paper contributes to the understanding of the mechanisms involved in the construction of exponential LbL multilayers and the knowledge that wine is associated with health-related benefit, LbL films from wine alternating with BSA can be used as a starting point for future investigations into processes based on the molecular interaction between wine and biological molecules that study the wine components immobile in films rather than wine in liquid phase.
This work was supported by CNPq and Capes (Brazil).
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