Research Article

A Novel Complex: A Quantum Dot Conjugated to an Active T7 RNA Polymerase

Figure 2

Separation of QD : T7RNAP complexes from free RNAPs. The Bio-T7 RNAPs are mixed with streptavidin-coated QDs thus forming QD : T7RNAP complexes. The suspension is mixed with an avidin resin to capture the remaining free Bio-T7 RNAPs. Once the suspension is centrifuged on a 0.45 μm filter only the QD : T7RNAP complexes and free QDs reach the bottom.
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