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Journal of Nanomaterials
Volume 2013, Article ID 536291, 9 pages
Research Article

Bovine Serum Albumin-Loaded Chitosan/Dextran Nanoparticles: Preparation and Evaluation of Ex Vivo Colloidal Stability in Serum

Centre for Drug Delivery Research, Faculty of Pharmacy, Universiti Kebangsaan Malaysia, Kuala Lumpur Campus, Jalan Raja Muda Abdul Aziz, 50300 Kuala Lumpur, Malaysia

Received 1 January 2013; Revised 22 March 2013; Accepted 7 April 2013

Academic Editor: Xing-Jie Liang

Copyright © 2013 Haliza Katas et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Chitosan (CS) nanoparticles have several distinct intrinsic advantages; however, their in vivo colloidal stability in biological fluids was not fully explored especially when carrying proteins. The present study aimed to investigate their colloidal stability using an ex vivo physiological model of fetal bovine serum (FBS) and human serum (HS). The stability of bovine-serum-albumin (BSA-) loaded nanoparticles was relatively higher in FBS than that in HS. Particle size of unloaded and BSA-loaded nanoparticles was statistically unchanged up to 24 h after incubation in FBS. However in HS, a significant increase in particle size from 144 ± 17 to 711 ± 22 nm was observed for unloaded nanoparticles and by 2.5-fold for BSA-loaded nanoparticle, at 24 h after incubation in HS. Zeta potential of both nanoparticles was less affected by the components in FBS compared to those in HS. A remarkable swelling extent was experienced for unloaded and BSA-loaded nanoparticles in HS, up to 54 ± 4% and 44 ± 5%, respectively. Morphology of unloaded and BSA-loaded nanoparticles was varied from smooth spherical and rod shape to irregular shape when incubated in FBS; however, form agglomerates when incubated in HS. These findings therefore suggest that HS is more reactive to cause colloidal instability to the chitosan nanoparticles compared to FBS.