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Journal of Nanomaterials
Volume 2013, Article ID 613638, 9 pages
http://dx.doi.org/10.1155/2013/613638
Research Article

Electrospun Hyaluronan-Gelatin Nanofibrous Matrix for Nerve Tissue Engineering

1Institute of Bioscience and Biotechnology, National Taiwan Ocean University, Keelung 20224, Taiwan
2Department of Life Sciences, National Taiwan Ocean University, Keelung 20224, Taiwan

Received 23 July 2013; Revised 25 September 2013; Accepted 26 September 2013

Academic Editor: In-Kyu Park

Copyright © 2013 Hau-Min Liou et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Schwann cells play a critical role in the repair of the peripheral nerve. The goal of this study was to fabricate electrospun gelatin (Gel) and hyaluronan-gelatin (HA-Gel) composite nanofibers to provide a suitable growth environment for Schwann cells. The fiber diameters of Gel, 0.5 HA-Gel, 1 HA-Gel, and 1.5 HA-Gel were 130 ± 30 nm, 294 ± 87 nm, 362 ± 129 nm, and 224 ± 54 nm, respectively. The biological performance of Gel and HA-Gel was evaluated using an in vitro culture of RT4-D6P2T rat Schwann cells. We found that the cell attachment and proliferation rates were not significantly different on these matrices. However, the Schwann cells displayed better organized F-actin on HA-Gel than on Gel. Moreover, the expression levels of several genes, including Nrg1, GFAP, and P0, were significantly higher on HA-Gel than on Gel. In addition, the levels of Nrg1 and P0 protein expression were also higher on the HA-Gel than on Gel. These results indicate that the hyaluronan-gelatin composite nanofibrous matrix could potentially be used in peripheral nerve repair.