Osteoconductivity and Biodegradability of Collagen Scaffold Coated with Nano-β-TCP and Fibroblast Growth Factor 2
Figure 5
Histological findings at 10 days. (a) Specimen receiving collagen sponge application. Cell and tissue ingrowth were rarely demonstrated in the collagen sponge. (b) Higher magnification of the framed area in (a). Collagen sponge was frequently compressed on the cranial bone. (c) Specimen receiving implantation of the nano-β-TCP/collagen scaffold. Ingrowth of regenerative tissue was observed at peripheral areas of implanted nano-β-TCP/collagen scaffolds on the skull of the rat. (d) Higher magnification of the framed area in (c). Macrophage-like cells (arrows) appeared around the agglutination of β-TCP particles (asterisks) of the scaffold. (e) Specimen receiving FGF2-loaded collagen sponge. New bone augmentation was highly promoted. (f) Higher magnification of the framed area in (e). Osteoblasts and osteocytes were found in newly formed woven bone. (g) Specimen receiving FGF2-loaded nano β-TCP/collagen scaffold. Formation of new bone as well as tissue ingrowth was prominently stimulated. (h) Higher magnification of the framed area in (g). Residual material was fractionated by newly formed connective tissue. Development of trabecular bone containing osteoblasts was evident. Abbreviations: NB, new bone; RM, residual material. Scale bars represent 1 mm ((a), (c), (e), (g)) and 100 μm ((b), (d), (f), (h)). Staining: HE.
