Osteoconductivity and Biodegradability of Collagen Scaffold Coated with Nano-β-TCP and Fibroblast Growth Factor 2
Figure 6
Histological findings at 35 days. (a) Group of collagen sponge implantation. Bone formation was localized near the preexisting bone, whereas residual material was obviously evident on the cranial bone. (b) Higher magnification of the framed area in (a). Cell ingrowth was sparse in the collagen sponge. (c) Specimen receiving implantation of nano β-TCP/collagen scaffold. New bone augmentation occurred and residual material was rarely observed. (d) Higher magnification of histological section with nano-β-TCP/collagen scaffold implantation. Residual large aggregation of β-TCP (asterisks) was mildly observed in newly formed bone and connective tissue. (e) Specimen receiving FGF2-loaded collagen sponge. Bone augmentation was observed at the material implantation area. (f) Higher magnification of framed area of (e). Residual collagen sponge was slightly detected in the connective tissue around the newly formed bone. (g) Specimen receiving FGF2-loaded nano-β-TCP/collagen scaffold. Advanced bone formation was demonstrated similarly to the FGF2-loaded collagen sponge. However, residual implanted material was scarcely detectable. (h) Control. There was little evidence of bone augmentation. Abbreviations: NB, new bone; RM, residual material. Scale bars represent 1 mm ((a), (c), (e), (g), (h)) and 100 μm ((b), (d), (f)). Staining: HE ((a)–(c), (e)–(h)) and Masson’s trichrome (d).
