In Vivo Study of Ligament-Bone Healing after Anterior Cruciate Ligament Reconstruction Using Autologous Tendons with Mesenchymal Stem Cells Affinity Peptide Conjugated Electrospun Nanofibrous Scaffold
Sirius red and immunohistochemical staining of ligament-bone insertion at 3 months after ACL reconstruction surgery. Sirius red staining observed by polarized light microscope indicated distribution of collagen type I (red) and III (green) of control group, PLLA reconstruction group, and BIO reconstruction group ((a)–(c)). Specific immunohistochemical staining showing distribution of collagen type II at ligament-bone insertion in 3 groups ((d)–(f)). Magnification: 100x. T: tendon; B: bone.