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Journal of Nanomaterials
Volume 2013, Article ID 842951, 8 pages
Research Article

Release Kinetics and Antibacterial Efficacy of Microporous -TCP Coatings

1Department of Orthopedics and Trauma Surgery, University of Freiburg Medical Center, Hugstetter Str. 55, 79106 Freiburg, Germany
2Faculty of Natural Sciences I, Institute of Pharmacy, Martin-Luther-University, Wolf-Langenbeck-Str. 4, 06120 Halle/Saale, Germany

Received 26 July 2013; Accepted 27 October 2013

Academic Editor: Krasimir Vasilev

Copyright © 2013 Michael Seidenstuecker et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Purpose. The aim of this study was to impregnate microporous β-TCP scaffolds with different antibiotic solutions and to determine their release behavior. Materials and Methods. We impregnated a β-TCP scaffold with antibiotics by using three methods: drop, dip, and stream coating with 120 mg/mL of antibiotic solution. After drying for 72 h at 37°C, 2 mL of distilled water was added to the antibiotic-coated plugs and incubated at 37°C. After defined time points (1, 2, 3, 6, 9, and 14 days), the liquid was completely replaced. The extracted liquid was analyzed by capillary zone electrophoresis and the Kirby Bauer disc diffusion test. For statistical analysis, we calculated a mean and standard deviation and carried out an analysis of variance using ANOVA. Results. The VAN and CLI release from the β-TCP scaffolds was rapid, occurring within 24 h with 89 ± 0.8% VAN and 90.4 ± 1.5% CLI regardless of the type of insulation. After six days, the VAN and CLI were completely released. All samples taken at later time points had a VAN or CLI concentration below the detection limit of 4 µg/mL. The released amounts of VAN and CLI within the first three days revealed antimicrobial activity.