Melatonin Nanoparticles Adsorbed to Polyethylene Glycol Microspheres as Activators of Human Colostrum Macrophages
Figure 5
Colostrum mononuclear cells stimulated with melatonin adsorbed to PEG microspheres were stained with Fluo-3 to assess intracellular release as described in Section 2. Immunofluorescence analyses were carried out by flow cytometry.