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Journal of Nanomaterials
Volume 2014, Article ID 124236, 7 pages
http://dx.doi.org/10.1155/2014/124236
Research Article

Novel Alginate-Gelatin Hybrid Nanoparticle for Drug Delivery and Tissue Engineering Applications

1Department of Life Sciences, Yeungnam University, Gyeongsan, Gyeongsangbuk-do 712-749, Republic of Korea
2Biomaterials Lab, Department of Nano, Medical & Polymer Materials, College of Engineering, Yeungnam University, 280 Daehak-ko, Gyeongsan, Gyeongsangbuk-do 712-749, Republic of Korea
3YU-ECI Medical Research Center, Yeungnam University, 280 Daehak-ko, Gyeongsan, Gyeongsangbuk-do 712-749, Republic of Korea

Received 30 June 2014; Revised 1 October 2014; Accepted 26 October 2014; Published 16 November 2014

Academic Editor: Gaurav Mago

Copyright © 2014 Eun Mi Lee et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Novel alginate-gelatin hybrid nanoparticles were fabricated using single oil in water (O/W) emulsification techniques. Physicochemical property of the particle was characterized using scanning electron microscopy and Fourier’s transmission infrared spectroscopy. Particle size was determined using zeta potential metastasize analyzer and was found to be in range of 400–600 nm. AGNPs were used for culturing human keratinocytes for two weeks to check biocompatibility of synthesized AGNPs. 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay showed increased metabolic activity of cells cultured on AGNPs in comparison to two-dimensional (2D) system (control). Cellular attachment on nanoparticle was further confirmed using SEM and 4′,6-diamidino-2-phenylindole staining. The drug release profile shows possible electrostatic bond between alginate and gelatin resulting in controlled release of drug from AGNPs. For the first time alginate-gelatin hybrid nanosystem has been fabricated and all results showed it can be used as potential system for delivery of drug and therapeutical agents to cells and can also be used for regenerative medicine applications.