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Journal of Nanomaterials
Volume 2014, Article ID 183815, 7 pages
Research Article

Characterization of the Casein/Keratin Self-Assembly Nanomicelles

College of Resources and Environment, Shaanxi University of Science and Technology, Xi’an 710021, China

Received 12 September 2013; Revised 16 January 2014; Accepted 19 January 2014; Published 25 February 2014

Academic Editor: Anchal Srivastava

Copyright © 2014 Su Xiao-Zhou et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Complex nanomicelles were made from casein and keratin through electrostatic self-assembly and transglutaminase fixation that was proved to be harmless and green. The complex nanomicelles were characterized by dynamic light scattering, scanning electron microscopy, atomic force microscopy, Fourier transform infrared spectroscopy, differential scanning calorimetry, and steady-state florescence. The results show that the complex nanomcelles acquired at the neutral pH in the mass ratio of casein to keratin 4 : 1 exhibit an anomalous sphere shape with uniform size which the diameter is about 40–70 nm. The complex nanomicelles in solution possess excellent dilution and storage stability due to the fixation and their high ζ-potential (22.8 mV). The complex nanomicelles are relatively hydrophilic and have a good potential for industrial application.