Structure and Identification of Solenin: A Novel Fibrous Protein from Bivalve Solen grandis Ligament

Table 1

Search result of FP identified by MALDI-TOF-TOF-MS, matching keratin, type II cytoskeletal 1 [Pan troglodytes], NCBI database ID: gi∣160961491.

Calculated mass	Observed mass	±ppm	Position	Peptide sequence^a,b
973.5312	973.572	42	396–403	IEISELNR
1033.516	1033.5568	39	479–487	TLLEGEESR
1065.5211	1065.5497	27	351–359	AQYEDIAQK
1092.5028	1092.5533	46	596–609	GSGGGSSGGSIGGR
1179.6005	1179.6487	41	372–381	YEELQITAGR
1277.71	1277.7668	44	468–478	LALDLEIATYR
1302.7151	1302.7572	32	339–350	SLDLDSIIAEVK
1340.6692	1340.719	37	360–371	SKAEAESLYQSK
1393.7322	1393.7858	38	273–284	TNAENEFVTIKK
1475.7489	1475.8197	48	207–218	WELLQQVDTSTR
1475.7853	1475.8197	23	195–206	FLEQQNQVLQTK
1539.7836	1539.8457	40	456–467	LLRDYQELM*NTK
1638.8599	1638.9277	41	181–194	SLNNQFASFIDKVR
1657.7928	1657.8588	40	13–29	SGGGFSSGSAGIINYQR
1708.7131	1708.7843	42	46–65	FSSCGGGGGSFGAGGGFGSR
1716.8511	1716.9194	40	413–427	QISNLQQSISDAEQR
1993.9825	1994.0464	32	618–637	SSGGSSSVKFVSTTYSGVTR
2150.0776	2150.1382	28	219–235	THNLEPYFESFINNLRR
2383.9517	2384.0327	34	514–544	GGGGGGYGSGGSSYGSGGGSYGSGGGGGGGR
3312.3079	3312.3784	21	545–583	GSYGSGGSSYGSGGGSYGSGGGGGGHGSYGSG SSSGGYR

aM*: oxidized methionine.
^bBold peptides are the repeat sequences.