Structure and Identification of Solenin: A Novel Fibrous Protein from Bivalve Solen grandis Ligament

Table 2

Search result of FP identified by MALDI-TOF-TOF-MS, matching keratin, type I cytoskeletal 9-like [Pongo  abelii], NCBI database ID: gi∣297716097.

Calculated mass	Observed mass	±ppm	Position	Peptide sequence^a,b
913.4084	913.4358	30	238–244	MTLDDFR
982.4337	982.4608	28	35–46	FSSSGGGGGGGR
1060.5634	1060.5917	27	229–237	TLLDIDNTR
1074.5215	1074.5509	27	5–13	QFSSSYLSR
1157.5909	1157.6195	25	255–265	QGVDADINGLR
1232.5978	1232.6311	27	14–29	SGGGGGGGLGSGGSIR
1235.5287	1235.556	22	47–59	FSSSSGYGGGSSR
1323.6725	1323.6975	19	245–254	IKFEM*EQNLR
1867.9219	1867.9476	14	326–340	TLNDM*RQEYEQLIAK
1966.0604	1966.0665	3	379–395	HGVQELEIELQSQLSKK
2705.1609	2705.1836	8	64–95	GGGGSFGYSYGGGSGGGFSASSLGGGFGGGSRGGGGSFGY; SYGGGSGGGFSASSLGGGFGGGSR

aM*: oxidized methionine.
^bBold peptides are the repeat sequences.