Research Article | Open Access
A. M. Gumel, M. S. M. Annuar, K. A. Ishak, N. Ahmad, "Carbon Nanofibers-Poly-3-hydroxyalkanoates Nanocomposite: Ultrasound-Assisted Dispersion and Thermostructural Properties", Journal of Nanomaterials, vol. 2014, Article ID 264206, 10 pages, 2014. https://doi.org/10.1155/2014/264206
Carbon Nanofibers-Poly-3-hydroxyalkanoates Nanocomposite: Ultrasound-Assisted Dispersion and Thermostructural Properties
The conductivity and high surface-to-volume ratio of carbon nanofibers (CNFs) composited with the medium-chain-length poly-3-hydroxyalkanoate (mcl-PHA) have attracted much attention as smart biomaterial. However, poor CNF dispersion leads to tactoid agglomerated composite with poor crystallite morphology resulting in inferior thermomechanical properties. We employed acoustic sonication to enhance the construction of exfoliated PHA/CNFs nanocomposites. The effects of CNF loading and the insonation variables (power intensity, frequency, and time) on the stability and microscopic morphology of the nanocomposites were studied. Sonication improved the dispersion of CNFs into the polymer matrix, thereby improving the physical morphology, crystallinity, and thermomechanical properties of the nanocomposites. For example, compositing the polymer with 10% w/w CNF resulted in 66% increase in crystallite size, 46% increase in micromolecular elastic strain, and 17% increase in lattice strain. Nevertheless, polymer degradation was observed following the ultrasound exposure. The constructed bionanocomposite could potentially be applied for organic electroconductive materials, biosensors and stimuli-responsive drug delivery devices.
Increasing environmental pollution and degradation as a result of the utilization of petrochemical derived plastics have intensified the search for commercially viable biodegradable polymers . Among the biopolymers are the microbial produced polyhydroxyalkanoates (PHA), which have received much attention due to their biocompatibility, biodegradability, and diverse structural composition, imparting onto them physicomechanical properties close to those of polyvinyl chloride and polyethylene terephthalate . These combinations of excellent physicochemical properties led to the increasing commercial exploitation of PHA in different niche applications spanning from biomedical, packaging, automotive, infrastructure, and aerospace to military [3, 4].
Despite their promising commercial potentials, PHAs such as those with monomeric composition of 3-hydroxybutyric acid were reported to exhibit brittleness, low heat distortion temperature, poor gas-barrier properties, limited processing malleability, and ductility [5, 6]. This in turn reduces the attractiveness and durability of the materials based on their strength, shelf lifetime, replacement, and maintenance cost. PHA modification and functionalization are expected to extend its quality or durability during application .
One approach for improving the thermomechanical stability of the biodegradable polymer is reinforcing the polymer using organic or inorganic nanofillers thereby extending their applications in aggressive environments where the neat polymers may fail [8–12]. Carbon nanoparticles were reported to exhibit high Young’s modulus and excellent electrical and thermal conductivities [13, 14]. Introduction of carbon fullerene into polymer as nanocomposites helped to improve the material’s physicomechanical properties such as polymer crystallization, gas-barrier property, thermomechanical strength, and electroconductivity surpassing those of the native biopolymers [14, 15]. Hence, PHA composites based on carbon nanoparticles filler have attracted much interest and are considered industrially important in such applications like microelectronics, membrane separation, fire-resistance fabrics, petrochemical specialized filters, tissue scaffolding, and drug delivery devices.
The physicomechanical properties of the composited material are highly dependent upon the degree of nanofibers dispersion in the organic polymer matrix. Heterogeneous dispersion of nanofillers into the polymer matrix resulted in weak structural stability of the composite . Insonation [17, 18], magnetron sputtering , spark plasma sintering , laser pyrolysis , and laser evaporation , and so forth, were employed to enhance the dispersion. In addition, surface functionalization was also reported to be an important way of improving the dispersion of nanoparticles into the polymer matrix [23–25]. While local turbulence due to the implosion of microbubbles from acoustic cavitation helps to disperse the carbon nanoparticles, the same high shear force could result in polymer degradation, thus affecting the thermomechanical properties of the fabricated material. Investigation on the thermomechanical stability of the fabricated polymer composite in relation to carbon nanoparticle loading and insonation variables is pertinent to the efficient process design and bionanocomposite properties.
In this study, the effects of carbon nanofibers (CNFs) loading and ultrasound irradiation variables on the physical morphology and thermomechanical stability of the poly-3-hydroxyalkanoates nanocomposites were studied. While there have been reports on the fabrication of PHA/carbon nanoparticles composites [13, 14, 26–29], the combined effects as investigated in this study on the properties of PHA/CNFs nanocomposite have yet to be reported.
2. Materials and Methods
Carbon nanofibers (CNFs) of optical Ø 200–600 nm were purchased from US Research Nanomaterials, Inc. Houston, USA (CAS number 99685-96-8). All analytical grades chemicals used in the study were purchased from Merck Millipore (Darmstadt, Germany).
Biodegradable medium-chain-length PHA with comonomeric composition of C4:0 to C10:0 and an average molecular weight (Mw) of 77.6 KDa was obtained from reported fermentation culture conditions , using Delftia tsuruhatensis Bet002 as a producer microorganism and oleic acid as a sole carbon and energy source.
2.2.1. Oxidation of CNF
The polymer nanocomposite fabrication was performed according to the method reported previously [29, 30], with slight modification. About 0.5 g of CNF was mixed in 20 mL of piranha solution containing 4 : 1 vol/vol of 97% sulfuric acid and 30% hydrogen peroxide and vortexed thoroughly. Then, the CNF mixture was oxidized by insonation at 40 kHz, 11.5 × 103 W m−2, 70°C for 1 h in a Multifrequency Ultrasonic Bath SB-300DTY (Ningbo Scientz Biotechnology Co., Ltd.; Zhejiang-China). The mixture was subsequently centrifuged at 9000 ×g to recover the oxidized CNF, which was followed by sequential washing with distilled water until the pH of the drained water was approximately neutral. The oxidized CNFs pellet was then dried in a vacuum oven at 50°C for 24 h in the presence of phosphoric anhydride and stored there prior to composite fabrication.
2.2.2. Polymer Composite Fabrication
The dried oxidized CNFs were then composited with PHA via solution dispersion method. A varied amount of oxidized CNFs powder based on percentage polymer weight (w/w) was suspended in 20 mL dichloromethane solution containing mg mcl-PHA in a capped vial and sonicated using DTY ultrasonic Bath (SB-300DTY) at 40 kHz, 4.7 × 103 W m−2, 50°C for 30 minutes. The solution was concentrated in rotary evaporator under reduced pressure to aliquot volume of about 2 mL and then casted in Petri dish into a thin film that was dried and kept in vacuo prior to further analyses. All reactions were carried out as described unless stated otherwise.
2.2.3. Characterization of Polymer Composite
(1) Field Emission Scanning Electron Microscopy (FESEM). The morphological characteristics of both the neat and the composited polymers were viewed in a high-resolution field emission scanning electron microscope (Quanta FEG 450) from FEI (Oregon, USA). The microscope was operated at high vacuum mode with an electron acceleration voltage of 5 kV and a working distance of about 10 mm. Thin films of neat polymer/polymer composite were mounted on brass stubs using double-sided cello tape and introduced into the viewing chamber of the instrument.
(2) FTIR-ATR Spectroscopy. A nondestructive attenuated total reflectance Fourier transform infrared spectra of the standard references and the composite polymer were recorded on Perkin-Elmer Spectrum 400 FT-IR and FT-NIR Spectrometer (Perkin-Elmer Inc., Wellesley, MA, USA) equipped with PIKE GladiATR hovering monolithic diamond ATR accessory (Pike technologies Inc., USA). Standard samples and their composites were placed on the monolithic diamond ATR probe and clamped against the diamond crystal plate using the force adapter. Thereafter, the samples were scanned over a range of 4000–400 cm−1 at 25°C as described previously .
(3) Wide Angle X-Ray Diffraction (WAXD) Analysis. The crystallite properties of both the pristine standards and the polymer composites were evaluated based on the diffractograms that were recorded on PANalytical Empyrean multipurpose X-ray diffractometer (PANalytical B.V.; The Netherlands) equipped with X-ray detector and anodic copper Kα1 radiation source ( Å) operated at 40 kV. Samples were scanned at 25°C over a range of 5° ≤ 2θ ≤ 70° with a step size of 0.026° s−1. The collected data of the diffraction pattern were analyzed using HighScore Plus version 3.0d software.
(4) Simultaneous Thermal Analysis. The thermal stability of the neat polymer and its composite was evaluated by simultaneous thermal analysis (STA) using Perkin Elmer STA 6000 machine (Perkin-Elmer Inc., Wellesley, MA, USA) operated on tandem differential scanning calorimetry (DSC) and thermogravimetric analysis (TGA) at nitrogen flow rate of 20 mL min−1. Samples (8 mg) were compressed in an aluminum boat and heated from 30°C to 550°C at a heating rate of 10°C min−1.
2.2.4. Viscosity Measurement
Viscosity of the polymer solution and that of the solvent was measured using a Sine-wave Vibro SV-10 viscometer (A and D Company, Japan), as previously reported .
2.2.5. Numerical Calculations
The ultrasonic power was calculated using calorimetric analysis described in (1) as previously reported , where is power (W), is the mass of the water (g), is the specific heat capacity of water (4.18 J g−1 K−1), is the temperature (K), and is the insonation time (s).
The calculated power is related to ultrasonic power intensity (W m−2) as in where represents the volumetric area (m2).
The ultrasonic degradation rate (s−1) is given by where is the initial polymer molecular weight and is polymer molecular weight at time .
The relative viscosity () and specific viscosity () are described by where and are the viscosities of polymer solution and the pure solvent, respectively.
The inherent viscosity () is described by where  is the polymer mass concentration in g L−1.
Dividing the specific viscosity with respective polymer mass concentration gives the reduced (intrinsic) viscosity (L g−1) as shown in Using Huggins’ empirical equations (see (7)), both the and were expressed as where the constants and were known to be a function of polymer, solvent, and temperature of the system; while  is a hypothetical viscosity at zero polymer mass concentration.
From (7), a plot of versus  and that of versus  yield a slope of and , respectively, with an intercept of  as depicted in Figure 1. Thus, from the plots,  value could be considered to be a true value, if the observed  from both Figures 1(a) and 1(b) are the same or .
Hence, the polymer viscosity molecular weight was determined according to semiempirical Mark-Houwink model (see (8)), where and are Mark-Houwink empirical constants. For universal PHA, these constants were empirically determined by Nerkar et al.  to be 1.66 × 10−4 and 0.689, respectively.
X-ray diffraction (XRD) pattern could provide an insight not only on the crystallite size and structure, but also on the internal strain-stress properties [35–38]. Hence, from the obtained XRD data, the crystallite size, internal elastic strains, and the percentage lattice strain were calculated using HighScore Plus ver 3.0d software and Williamson-Hall (see (10)) empirical equations [36–38]. Consider where is the crystallite size, is Debye-Scherrer’s shape factor constant denoted by 0.9 [36–38], is the Cukα radiation wave length, and is the full width at half maximum (FWHM) intensity (referring to the peak-width measured at position halfway from the peak’s apex, roughly donated by the difference between the 2θ base line points of the peak as highlighted by Cullity ).
Polymer thermal degree of crystallinity () is calculated according to (11) as described in literatures [27, 40]. Consider where is the STA melting enthalpy of the PHA, is the melting enthalpy of the standard crystalline PHBV (146.6 J g−1), and is the PHA mass fraction in the composite.
3. Results and Discussion
3.1. Characterization of Polymer Nanocomposite
The FESEM micrograph of the polymer composites showed the dispersed carbon nanotubes within the polymer matrix (Figure 2). It can be observed that in the ultrasound-assisted PHA/CNF fabrication, acoustic generated turbulence resulted in an exfoliated morphological characteristic in the polymer composite for both lower (Figure 2(a)) and higher (Figure 2(b)) CNF loadings. This indicated well-dispersed CNTs within the PHA matrix. On the other hand, conventional melt-mixed PHA/CNF composite was observed to produce similar exfoliated morphology for composite at lower CNF loading (0.5%) (Figure 2(c)). At higher CNF loading (5%), tactoid aggregation morphology was observed in the composite prepared via solvent dispersion route (Figure 2(d)).
The oxidation of the carbon nanofibers and successful dispersion of CNTs within the polymer matrices were indicated by the FTIR-ATR spectra as shown in Figure 3. The observed prominent FTIR absorption at 3394 cm−1 in the oxidized CNF sample signified the presence of hydroxyl (–OH) group. This band was observed to be highly reduced in composite polymer sample, indicating successful dispersion of the CNF within the polymer matrix. The absorption was also found to be less prominent in both the pristine CNF and neat PHA samples.
Absorption at 2925 and 2845 cm−1 was attributed to both asymmetric CH3 and symmetric CH2 vibrations in the samples, respectively. The presence of carbonyl ester bond in neat PHA sample was assigned to the absorption at 1735 cm−1. This vibration was shifted to 1727 cm−1 in polymer-CNF composite sample signifying an increase in crystallinity. This observation was found to be in agreement with reported shifting of the said band in polyester carbonyl groups due to the increase in crystallinity . The absorption band at 1633 cm−1 belongs to C=C [42, 43]. The series of bands between 1543 and 1450 cm−1 were reported to be due to CH bending of methyl and methylene groups ; this region is also known to overlap with C–O–C stretching vibrations [43, 44]. Furthermore, in the pristine CNF samples, the sharp absorptions at 1545 and about 823 cm−1 illustrated the reported normal vibration of carbon nanoparticles phonon modes E1u and A2u, respectively [43, 45]. In the oxidized CNF, the absorption at 1367 cm−1 is due to the C–OH stretching vibrations , whereas series of absorptions in all spectra at 1276–736 cm−1 was reported to be due to C–O stretching vibrations [42, 43]. In fact, the relative intensity and partial separation of bands at 1250–950 cm−1 were suggested to be dependent of the amounts of OH deformation and C–O stretching combination in surface of phenolic rings and aromatic carboxylic acids . In agreement with this observation, the CNF oxidation was strongly indicated in this study due to the presence of sharp and highly intense absorption at 1056 cm−1 wavelength. This band was observed to be less prominent in pristine CNF sample, whereas the intensity of the peak was significantly reduced in the polymer composite sample probably due to dispersion effect.
The crystalline nature of the tested samples is shown in Figure 4. In the CNF diffractogram, an intense crystalline peak was observed at 2θ value of 27.4°, a typical characteristics of graphite hexagonal peak at (002) plane [42, 45]. The -spacing of this peak was found to stand at 0.33 nm with corresponding percentage lattice strain of 2.6% and micromolecular elastic strain of 0.3. Similar -spacing value based on peak 002 reflection was observed by Vinayan et al.  in multiwalled carbon nanotube (0.336 nm) and graphite sheets (0.334 nm). For oxidized CNF, this intense crystalline peak (002) was found to be shifted to lower values of 2θ (25.9°) with corresponding increase in the -spacing to 0.347 nm and lattice strain 3.88% and elastic strain of 0.46 with an appearance of broader peak at 43.8° (Figure 4). The decrease in the 2θ angle and the corresponding increase in interfiber -spacing were suggested to be due to the dispersion of oxygen containing functional groups, which could also caused the observed increase in elastic and lattice strain. The observed broadening of the peak 101 reflection was previously linked to the lattice distortion along the -axis .
In comparison to the pristine CNF, the standard PHA was observed to display the typical polymeric crystallite reflections at (020), (110), (111), and (040) planes (Figure 4). Additionally, the 040 reflection peak in the neat PHA was observed to be broader due to the presence of less perfect crystal structure in the neat PHA. Intercalating the oxidized CNF with PHA resulted in the intense appearance of 002 reflection at 27.5° on 2θ scale. In contrast to pristine CNF, the peak in this composite was observed to exhibit a decrease in the -spacing to 0.32 nm indicating utilization of oxygen functional groups to form polymer-CNF dispersion. In addition, the 002 reflection peak was found to be less intense in PHA/CNF composite difractogram as compared to the observed 002 peak in the pristine CNF. This was attributed to the strong hydrogen bonding between the carboxyl groups of the organic polymer matrix and the hydroxyl groups of the CNF .
It is noted that compositing the PHA with CNF at different fractions increased the PHA crystallinity due to the observed increase in crystallite size, micromolecular elastic strain, and percentage lattice strain. For example, in Table 1, the crystallite size at 020 reflection was observed to be increased from 4.91 nm in neat PHA to 5.15 nm in PHA composite containing 0.5% CNF. It has been documented that molecular crystallization is highly dependent on the rate of nucleation and crystal growth rate . The observed elevated crystallite size and the increase in peak intensity (Figure 4) strongly indicated the crystallization effect of CNF on PHA. Similar observations were reported on PHBV composites a [40, 46]. Shan et al.  reported that the addition of carbon nanotubes greatly enhances the nucleation rate of PHBV resulting in efficient crystallization. Similarly, addition of multiwalled carbon nanotubes also resulted in increased crystallization in PHBV .
To substantiate the hypothesis, the crystallite size was observed to keep increasing with the increase in CNF content up to 7% (14.43 nm) after which further addition resulted in poor crystallization (Table 1). In fact, compositing the neat PHA with 10% oxidized CNF resulted in a lower crystallite size (14.22 nm) with corresponding decrease in elastic strain (2.53) and percentage lattice strain (2.62%). This could be due to the reported interference in the macromolecular continuity of the polymer such as that observed in polyethylene  and PHBV  matrices caused by higher loading of carbon nanotubes in polymer composite. This affects the mobility of the polymer chains resulting in gain boundaries and crystallite distortion.
The observed increase in polymer crystallinity as a result of CNTs composition was corroborated by the data obtained from simulteneous thermal analysis (Table 1). From the STA thermograms, the degree of crystallinity was calculated according to empirical model given in (11). In accordance with observed increased in crystallite size, the thermal stability of the polymer composite based on melting temperature () and degradation temperature () as well as the degree of crystallinity were all found to be increased upon dispersion of the CNF into the organic polymer matrix in a CNF fraction dependent fashion (Table 1). Compositing the neat PHA with 7% (w/w) CNF was found to elevate the crystallinity by about 58.2% resulting in corresponding increase of and from 163.6°C and 272.2°C to 183.5°C and 331.2°C, respectively. In agreement with earlier WAXD observation, polymer composited samples of higher CNF loading (10% w/w) were observed to exhibit lower and . This could probably be attributed to the crystallite distortion as a result of higher CNF loading. Thus, it could suffice to say that in this study, at higher CNF loading incurs similar effect, which resulted in the observed decrease in the crystallite size and crystallinity.
3.2. Effects of Insonation Variables (Power Intensity, Frequency, and Time) on the PHA/CNF Nanocomposite Thermophysical Properties and Stability
The effects of sonication intensity on the dispersion of 0.5% CNTs (w/w) were studied over a range of W m−2 at 40 KHz, 50°C for 30 minutes. At power intensity lower than W m−2, the tactoid aggregated morphology was clearly evident (Figures 5(a) and 5(b)). The characteristic exfoliated morphology of PHA-CNF composite was observed at and W m−2 power intensities (Figure 5), indicative of well-dispersed CNTs. However, at higher intensities such as W m−2 reduction in polymer molecular weight was observed possibly due to partial degradation of the polymer as a result of high turbulent generated by cavitation implode (Table 2). This could explain the observation made in the sample of nanocomposite irradiated at higher power intensity (Figure 5(d), inset) where a segment appeared to have melted due to the heating effect by the applied electron voltage of FESEM. The partial degradation of the polymer caused by high insonation intensity could be responsible in lowering the melting temperature of PHA at the particular segment. Similar melting effect, however, was not observed in the other samples of nanocomposite irradiated at lower power intensity (Figures 5(a)–5(c), insets). Notwithstanding the observed degradation, the improved CNF dispersion with increasing power intensity was observed to increase the polymer thermal stability and crystalline properties (Table 2). Increasing the power intensity from to W m−2 resulted in an observed decrease in from 75.2 to 59.8 kDa. The possible mechanism of the polymer degradation with increasing sonication power was reported previously .
Specifically, the increase in sonication power intensity from W m−2 resulted in increased dispersion of the CNFs incurring the corresponding increase in , , and from 132.2°C, 298.4°C, and 71.2% to 160.1°C, 326.4°C, and 85.4%, respectively (Table 2). Based on the trend of increase in the and with power intensity (Table 2), it was expected that the increase in and at W m−2 would be significantly higher than the recorded values. However, this was not the case. As the observation was attributed to the partial polymer degradation resulting in lower , and, hence, small increase in the and at W m−2.
When the insonation frequency was varied from 59 to 25 KHz for 30 minutes irradiation period, reduction in the crystallinity of the polymer composite was observed (Figure 6(a)). This was attributed to the increase in polymer degradation (hence control crystallinity) with a decrease in applied ultrasonic frequency. There is a wealth of experimental information that showed increased polymer degradation with a decrease in insonation frequency [49, 50]. At 40 kHz sonication frequency, negligible decrease and increase in the viscosity average molecular weight (), and crystallinity () were observed, respectively, when irradiation time was prolonged from 30 minutes to 40 minutes. After 40 minutes and above of acoustic irradiation, no significant decrease nor increase in the weight average molecular weight () and crystallinity () was observed, respectively (Figure 6(b)). This indicated that irradiation time could be limited to 30 minutes.
The application of sonication enhanced the dispersion of CNFs into the polymer matrix, thereby improving the physical morphology, crystallinity, and thermomechanical properties of the nanocomposites. Controlling the sonication variables such as power intensity, frequency, and time as well as the fraction of CNFs loading could result in efficient fabrication of smart and biocompatible nanocomposites that could find applications in organic electroconductive materials, biosensors, and drug delivery devices.
Conflict of Interests
The authors declare that there is no conflict of interests regarding the publication of this paper.
We acknowledged University of Malaya for providing the research Grants nos. BK014-2014, RG024-2012A, UM.C/625/1/HIR/MOHE/05, and PV036-2012A.
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