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Journal of Nanomaterials
Volume 2014, Article ID 351848, 7 pages
http://dx.doi.org/10.1155/2014/351848
Research Article

Time-Evolution Contrast of Target MRI Using High-Stability Antibody Functionalized Magnetic Nanoparticles: An Animal Model

1Department of Surgery & Hepatitis Research Center, National Taiwan University Hospital, Taipei 100, Taiwan
2Graduate Institute of Clinical Medicine, College of Medicine, National Taiwan University, Taipei 100, Taiwan
3MagQu Co., Ltd., New Taipei City 231, Taiwan
4Institute of Electro-Optical Science and Technology, National Taiwan Normal University, Taipei 116, Taiwan
5Department of Internal Medicine, National Taiwan University Hospital, College of Medicine, National Taiwan University, Taipei 100, Taiwan
6Department of Primary Care Medicine, National Taiwan University, Taipei 100, Taiwan
7Department of Material Engineering, Kun Shan University, Tainan City 710, Taiwan
8Center for Molecular Imaging and Translational Medicine, Xiamen University, Xiamen 361, China
9Graduate Institute of Electronics Engineering, National Taiwan University, Taipei 106, Taiwan
10Department of Electro-Optical Engineering, Kun Shan University, Tainan City 710, Taiwan

Received 30 May 2014; Accepted 23 July 2014; Published 27 August 2014

Academic Editor: Yali Cui

Copyright © 2014 K. W. Huang et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

In this work, high-quality antibody functionalized Fe3O4 magnetic nanoparticles are synthesized. Such physical characterizations as particle morphology, particle size, stability, and relaxivity of magnetic particles are investigated. The immunoreactivity of biofunctionalized magnetic nanoparticles is examined by utilizing immunomagnetic reduction. The results show that the mean diameter of antibody functionalized magnetic nanoparticles is around 50 nm, and the relaxivity of the magnetic particles is 145 (mM·s)−1. In addition to characterizing the magnetic nanoparticles, the feasibility of using the antibody functionalized magnetic nanoparticles for the contrast medium of target magnetic resonance imaging is investigated. These antibody functionalized magnetic nanoparticles are injected into mice bearing with tumor. The tumor magnetic-resonance image becomes darker after the injection and then recovers 50 hours after the injection. The tumor magnetic-resonance image becomes the darkest at around 20 hours after the injection. Thus, the observing time window for the specific labeling of tumors with antibody functionalized magnetic nanoparticles was found to be 20 hours after injecting biofunctionalized magnetic nanoparticles into mice. The biopsy of tumor is stained after the injection to prove that the long-term darkness of tumor magnetic-resonance image is due to the specific anchoring of antibody functionalized magnetic nanoparticles at tumor.