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Journal of Nanomaterials
Volume 2014, Article ID 417305, 12 pages
Review Article

Green Nanobiotechnology: Factors Affecting Synthesis and Characterization Techniques

School of Biotechnology, Yeungnam University, Gyeongsan, Gyeongbuk 712-749, Republic of Korea

Received 13 October 2014; Accepted 1 December 2014; Published 17 December 2014

Academic Editor: Wei Chen

Copyright © 2014 Jayanta Kumar Patra and Kwang-Hyun Baek. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Nanobiotechnology is gaining tremendous impetus in this era owing to its ability to modulate metals into their nanosize, which efficiently changes their chemical, physical, and optical properties. Accordingly, considerable attention is being given to the development of novel strategies for the synthesis of different kinds of nanoparticles of specific composition and size using biological sources. However, most of the currently available techniques are expensive, environmentally harmful, and inefficient with respect to materials and energy use. Several factors such as the method used for synthesis, pH, temperature, pressure, time, particle size, pore size, environment, and proximity greatly influence the quality and quantity of the synthesized nanoparticles and their characterization and applications. Additionally, characterization of the synthesized nanoparticles is essential to their potential use in various drug delivery and biomedical applications. The present review highlights various parameters affecting the synthesis of nanoparticles by green nanobiotechnology and different techniques used for characterizing the nanoparticles for their potential use in biomedical and environmental applications.