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Journal of Nanomaterials
Volume 2014, Article ID 576082, 7 pages
http://dx.doi.org/10.1155/2014/576082
Research Article

Shape-Controlled Generation of Gold Nanoparticles Assisted by Dual-Molecules: The Development of Hydrogen Peroxide and Oxidase-Based Biosensors

State Key Laboratory of Food Science and Technology, School of Food Science and Technology, Jiangnan University, Wuxi 214122, China

Received 29 June 2014; Accepted 5 September 2014; Published 13 October 2014

Academic Editor: Marinella Striccoli

Copyright © 2014 Chifang Peng et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

With the assist of dual-molecules, 2-(N-morpholino)ethanesulfonic acid (MES) and sodium citrate, gold nanoparticles (GNPs) with different shapes can be generated in the H2O2-mediated reduction of chloroauric acid. This one-pot reaction can be employed to sensitively detect H2O2, probe substrates or enzymes in oxidase-based reactions as well as prepare branched GNPs controllably. By the “naked eye,” 20 μM H2O2, 0.1 μM glucose, and 0.26 U/mL catalase could be differentiated, respectively. By spectrophotometer, the detected limits of H2O2, glucose, and catalase were 1.0 μM, 0.01 μM, and 0.03 U/mL, respectively, and the detection linear ranges for them were 5.0–400 μM, 0.01–0.3 mM, and 0.03–0.78 U/mL, respectively. The proposed “dual-molecules assist” strategy probably paves a new way for the fabrication of nanosensors based on the growth of anisotropic metal nanoparticles, and the developed catalase sensor can probably be utilized to fabricate ultrasensitive ELISA methods for various analytes.