Titanium Oxide Nanotube Surface Topography and MicroRNA-488 Contribute to Modulating Osteogenesis
Figure 3
miR-488 involved in osteoblastic differentiation of MC3T3-E1 cells on TiO2 nanotube surface. (a) MC3T3-E1 cells were cultured on titanium oxide nanotube having various diameters (30, 50, 70, and 100 nm), titanium oxide (TiO2), or culture dish (Cd). The expression of miR-488 was measured at day 2. RQ of cells on culture dish (Cd) was used as a control to measure fold change. (b) MC3T3-E1 cells were cultured with antisense oligonucleotides of miR-488 (anti-miR-488) or a scrambled PNA-based ASO (Con). ALP expression was measured using real-time PCR and cell adhesion was assayed. RQ of control cells on culture dish (Cd) was used as a control to measure fold change. (c) MC3T3-E1 cells were cultured on titanium oxide nanotube having various diameters (30, 50, 70, and 100 nm), titanium oxide (TiO2), or culture dish (Cd) and treated with antisense oligonucleotides of miR-488 (anti-miR-488) or a scrambled PNA-based ASO (Sc). ALP expression was measured using real-time PCR. RQ of cells treated with Sc was used as a control to measure fold change. Representative data is shown (). The mean is plotted and the error bars represent 95% CI (lower/upper limit). *.
