Titanium Oxide Nanotube Surface Topography and MicroRNA-488 Contribute to Modulating Osteogenesis
Figure 4
MMP-2 modulation by miR-448 affects osteoblastic differentiation of MC3T3-E1 cells on TiO2 nanotube surface. (a) MC3T3-E1 cells were cultured on titanium oxide nanotube having various diameters (30, 50, 70, and 100 nm), titanium oxide (TiO2), or culture dish (Cd) and the expression of MMP-2 was measured using real-time PCR. RQ of cells on culture dish (Cd) was used as a control to measure fold change. (b) MC3T3-E1 cells were treated with bafilomycin A1 or GM6001 and the expression of ALP was measured using real-time PCR. RQ of cells on flat titanium oxide was used as a control to measure fold change. (c) MC3T3-E1 cells were cultured on titanium nanotube having various diameters (30, 50, 70, and 100 nm) and treated with antisense oligonucleotides of miR-488 (anti-miR-488) or a scrambled PNA-based ASO (Con). Expression of MMP-2 was measured. RQ of cells on flat titanium oxide was used as a control to measure fold change. Representative data is shown (). The mean is plotted and the error bars represent 95% CI (lower/upper limit). *.
