The Promotion of Human Neural Stem Cells Adhesion Using Bioinspired Poly(norepinephrine) Nanoscale Coating
Figure 4
Cellular adhesion on pNE interfaces. (a) The percentage of cells was attached to each substrate, indicating the ratio of the number of cells remaining on each substrate to the initial number prior to seeding. To quantify the cell number, hNSCs were rinsed twice with PBS after 2 hours of culture and trypsinized; the cell number was manually counted using a hemocytometer. The symbol indicates a significant difference compared to each (). (b) Fluorescence images of hNSCs were adhered to each substrate. After 2 hours of culture, hNSCs were rinsed twice with PBS, and the nuclei were stained with DAPI to visualize the cells on each substrate. The scale bar indicates 10 micrometers.
