The Promotion of Human Neural Stem Cells Adhesion Using Bioinspired Poly(norepinephrine) Nanoscale Coating
Figure 6
Cellular patterns created using pNE interfaces. (a) Macroscale cellular patterns were created using a micropipette. The “Y” shape of the pNE interfaces was generated with a pipette, and hNSCs were seeded onto the entire TCP substrate. After 2 hours of culture, the loose cells were removed by rinsing with PBS, and the adherent cells were stained with Hoechst dye and imaged using fluorescence microscopy. To visualize the entire cellular pattern, individual images were collected and combined to obtain the entire image. The scale bar indicates 1 mm. (b) Microscale cellular patterns were created with a PDMS stamp. The PDMS stamp was immersed in pNE solution and placed on a TCP plate. The substrate was rinsed with PBS; the cells were seeded onto the entire substrate, rinsed with PBS after 2 hours of culture, and imaged. The nuclei were stained using Hoechst dye. The scale bar indicates 100 micrometers.
