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Journal of Nanomaterials
Volume 2014 (2014), Article ID 802596, 7 pages
http://dx.doi.org/10.1155/2014/802596
Research Article

Systematic Evaluation and Mechanistic Investigation of Antioxidant Activity of Fullerenols Using β-Carotene Bleaching Assay

Division of Applied Chemistry, Graduate School of Engineering, Osaka University, 2-1 Yamadaoka, Suita, Osaka Prefecture 565-0871, Japan

Received 27 June 2014; Accepted 12 August 2014; Published 28 August 2014

Academic Editor: Naoki Kishi

Copyright © 2014 Hiroshi Ueno et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Antioxidant activity of hydroxylated fullerenes, so-called fullerenols, against lipid peroxyl radical was evaluated by β-carotene bleaching assay. All samples showed moderate to high antioxidant activity (%AOA), especially for C60(OH)12 (70.1) and C60(OH)44 (66.0) as compared with 8, 24, 26, and 36 hydroxylated ones (31.7–62.8). The detection of the possible products was conducted in the model reaction of both fullerenols and C60 with methyl linoleate by MALDI-TOF-MS. These results suggested that the two possible mechanisms, such as C-addition to double bonds and H-abstraction from –OH groups, are involved in the present radical scavenging reaction.