Systematic Evaluation and Mechanistic Investigation of Antioxidant Activity of Fullerenols Using β-Carotene Bleaching Assay
Figure 2
(a) Time course of the discoloration of β-carotene induced by autoxidation of linoleic acid under air at 50°C in the absence (solid line for control) or the presence of fullerenols (dotted line for C60(OH)12 and dashed line for C60(OH)8 in 10 μM) by monitoring of UV absorbance at 460 nm. (b) Plots of the ratio of β-carotene bleaching (discoloration) rates in the presence () or absence () of fullerenol / versus the ratio of concentration [fullerenol]/[β-carotene] for various fullerenols: marked by square for C60(OH)12, triangle for C60(OH)8, and circle for C60(OH)26. The slope of each linear regression line corresponds to the relative radical scavenging rate constant of fullerenols relative to β-carotene (=/). The dotted horizontal line indicates the value in the absence of antioxidant as a control ( = at any concentration; = 0).
