Label-Free Dengue Detection Utilizing PNA/DNA Hybridization Based on the Aggregation Process of Unmodified Gold Nanoparticles

Table 3

Ratio concentrations of PNA/DNA in the hybridization process. All the assay conditions were in the presence of AuNPs solution. (Assay 1 for AuNPs, 2 for AuNPs/PNA, and 3 to 8 for AuNPs with the complex of PNA/DNA, with different ratios.)

Assay	PNA probe (𝜇M)	Complementary DNA (𝜇M)	Ratio (PNA : DNA)
1	—	—	—
2	12	—	—
3	12	0.12	1 : 0.01
4	12	0.36	1 : 0.03
5	12	0.6	1 : 0.05
6	12	1.2	1 : 0.1
7	12	6	1 : 0.5
8	12	12	1 : 1