Cell cycle distributions of MCF-7 cells were performed by flow cytometric detection. The distributions of different solutions treated with free ORI and ORI-loaded SLNs at the concentrations of 20 μM and 10 μM for 24 h were presented and compared with control and blank SLNs (a). The percentages of G2/M cells treated with free ORI and ORI-loaded SLNs at the concentrations of 20 μM and 10 μM (b) and the percentages of cells on G1/G0 (c) and S (d) were also presented. * versus control.