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Journal of Nanomaterials
Volume 2014, Article ID 903691, 9 pages
Research Article

Preparation and Characterization of a Collagen-Liposome-Chondroitin Sulfate Matrix with Potential Application for Inflammatory Disorders Treatment

1National Institute of Research and Development for Biological Sciences, No. 296, Splaiul Independentei, Sector 6, 060031 Bucharest, Romania
2Institute of Biochemistry of the Romanian Academy, No. 296, Splaiul Independentei, Sector 6, 060031 Bucharest, Romania
3Faculty of Biology, University of Bucharest, No. 91-95, Splaiul Independentei, Sector 5, 050095 Bucharest, Romania

Received 19 March 2014; Accepted 28 April 2014; Published 18 May 2014

Academic Editor: Chunyi Zhi

Copyright © 2014 Oana Craciunescu et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Smart drug delivery systems with controllable properties play an important role in targeted therapy and tissue regeneration. The aim of our study was the preparation and in vitro evaluation of a collagen (Col) matrix embedding a liposomal formulation of chondroitin sulfate (L-CS) for the treatment of inflammatory disorders. Structural studies using Oil Red O specific staining for lipids and scanning electron microscopy showed an alveolar network of nanosized Col fibrils decorated with deposits of L-CS at both periphery and inner of the matrix. The porosity and density of Col-L-CS matrix were similar to those of Col matrix, while its mean pore size and biodegradability had significantly higher and lower values (), respectively. In vitro cytotoxicity assays showed that the matrix system induced high cell viability and stimulated cell metabolism in L929 fibroblast cell culture. Light and electron micrographs of the cell-matrix construct showed that cells clustered into the porous structure at 72 h of cultivation. In vitro diffusion test indicated that the quantity of released CS was significantly lower () after embedment of L-CS within Col matrix. All these results indicated that the biocompatible and biodegradable Col-L-CS matrix might be a promising delivery system for local treatment of inflamed site.