Impact of Silver and Iron Nanoparticle Exposure on Cholesterol Uptake by Macrophages

Figure 4

Measurement of NP uptake by assessment of changes in mean side scatter shift (SSC) via flow cytometry. (a) Macrophages were exposed to 20 nm AgNP, 110 nm AgNPs, or 20 nm Fe₃O₄ NPs at a concentration of 25 μg/mL for 2 h and assessed for changes in side scatter shift (SSC). (b) Macrophages were exposed to 20 nm AgNP, 110 nm AgNPs, or 20 nm Fe₃O₄ NPs at a concentration of 25 μg/mL for 24 h in either serum-free media or serum-free media containing cholesterol (20 μg/mL). SSC values of macrophages exposed to NPs were normalized to control macrophages to produce a fold change. Values are expressed as mean ± SEM (/group). indicates significant difference from controls (). indicates significant difference from NP exposure in serum-free media ().

(a)

(b)