Impact of Silver and Iron Nanoparticle Exposure on Cholesterol Uptake by Macrophages

Figure 8

Alteration in macrophage surface receptor expression of scavenger receptor-B1 (SR-B1). (a) Macrophages were exposed to 20 nm AgNP, 110 nm AgNPs, or 20 nm Fe₃O₄ NPs at a concentration of 25 μg/mL for 2 h and assessed for changes in SR-B1 cell surface expression by flow cytometry. (b) Macrophages were exposed to 20 nm AgNP, 110 nm AgNPs, or 20 nm Fe₃O₄ NPs at a concentration of 25 μg/mL for 24 h in either serum-free media or serum-free media containing cholesterol (20 μg/mL) and assessed for changes in SR-B1 cell surface expression. The mean fluorescent signal from no stain controls was subtracted from SR-B1 stained samples to correct for any background autofluorescence. Values are expressed as mean ± SEM (/group). indicates significant difference from controls (). # indicates significant difference from NP exposure in serum-free media ().

(a)

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