Induction of Heat Shock Protein-72 by Magnetic Nanofluid Hyperthermia in Cultured Retinal Ganglion Cells for Neuroprotective Treatment in Glaucoma

Figure 6

Immunofluorescent staining of HSP72. (a) Control group 1, without SPNPs and without magnetic field application. (b) Control group 2, without SPNPs but with magnetic field application ( kHz and  Oe). (c) Control group 3, without magnetic field application but incubated with SPNPs (500 μg/mL). (d) RGCs incubated with SPNPs (500 μg/mL) and with the AC magnetic field applied ( kHz and  Oe). HSP72 was not induced in the control groups 1, 2, and 3 but was successfully induced in RGCs incubated with SPNPs when an adequate magnetic field was applied.

(a)

(b)

(c)

(d)