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Journal of Nanomaterials
Volume 2015, Article ID 150394, 8 pages
Research Article

Comparison of the Effect of Sol-Gel and Coprecipitation Routes on the Properties and Behavior of Nanocomposite Chitosan-Bioactive Glass Membranes for Bone Tissue Engineering

1NDBio Research Group, Department of Metallurgical and Materials Engineering, Universidade Federal de Minas Gerais, 253 31270-901 Belo Horizonte, MG, Brazil
2Department of Chemistry, Centro Federal de Educação Tecnológica de Minas Gerais, 30 421-169 Belo Horizonte, MG, Brazil
3Department of Chemistry, Universidade Federal de Minas Gerais, 253 31270-901 Belo Horizonte, MG, Brazil

Received 13 April 2014; Revised 16 August 2014; Accepted 25 September 2014

Academic Editor: Sharmila M. Mukhopadhyay

Copyright © 2015 Elke M. F. Lemos et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Recent studies in tissue engineering have highlighted the importance of the development of composite materials based on biodegradable polymers containing bioactive glasses, in particular, composites for high load support and excellent cell viability for potential application in bone regeneration. In this work, hybrid composite films were obtained by combining chitosan with bioactive glass in solution form and in nanoparticle dispersion form obtained by the two different synthesis routes: the sol-gel method and coprecipitation. The bioactive glass served both as a mechanical reinforcing agent and as a triggering agent with high bioactivity. The results of in vitro assays with simulated body fluid demonstrated the formation of a significant layer of fibrils on the surface of the film, with a typical morphology of carbonated hydroxyapatite, reflecting induction of a favorable bioactivity. Maximum tensile stress increased from 42 to 80 MPa to the sample with 5% wt bioactive glass. In addition, samples containing 5% and 10% wt bioactive glass showed a significant increase in cell viability, 18 and 30% increase compared to the control group. The samples showed significant response, indicating that they could be a potential material for use in bone regeneration through tissue engineering.