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Journal of Nanomaterials
Volume 2015, Article ID 183438, 11 pages
http://dx.doi.org/10.1155/2015/183438
Research Article

Rapid and Sensitive Detection of Lung Cancer Biomarker Using Nanoporous Biosensor Based on Localized Surface Plasmon Resonance Coupled with Interferometry

1School of Electronics Engineering, College of IT Engineering, Kyungpook National University, Daegu 702-701, Republic of Korea
2Gyeongbuk Institute of IT Convergence Industry Technology, Gyeongsangbuk-do 712-832, Republic of Korea
3Center for Functional Devices Fusion Platform, Kyungpook National University, Daegu 702-701, Republic of Korea
4Department of Biomedical, School of Medicine, Dankook University, Chungnam 330-714, Republic of Korea
5Department of Electronics Engineering, Kyungil University, Gyeongsangbuk-do 712-702, Republic of Korea

Received 22 May 2015; Accepted 15 July 2015

Academic Editor: Anh-Tuan Le

Copyright © 2015 Jae-Sung Lee et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

We propose a nanobiosensor to evaluate a lung cancer-specific biomarker. The nanobiosensor is based on an anodic aluminum oxide (AAO) chip and functions on the principles of localized surface plasmon resonance (LSPR) and interferometry. The pore-depth of the fabricated nanoporous AAO chip was 1 µm and was obtained using a two-step electrochemical anodization process. The sensor chip is sensitive to the refractive index (RI) changes of the surrounding medium and also provides simple and label-free detection when specific antibodies are immobilized on the gold-deposited surface of the AAO chip. In order to confirm the effectiveness of the sensor, the antibodies were immobilized on the surface of the AAO chip, and the lung cancer-specific biomarker was applied atop of the immobilized-antibody layer using the self-assembled monolayer method. The nanoporous AAO chip was used as a sensor system to detect serum amyloid A1, which is a lung cancer-specific biomarker. The specific reaction of the antigen-antibody contributes to the change in the RI. This in turn causes a shift in the resonance spectrum in the refractive interference pattern. The limit of detection (LOD) was found to be 100 ag/mL and the biosensor had high sensitivity over a wide concentration range.