Effect of Lipophilic Bismuth Nanoparticles on Erythrocytes

Figure 5

BisBAL nanoparticles on genomic DNA of erythrocytes by Comet Assay and fluorescence microscopy. To determine the possible damage in genomic DNA of erythrocytes after exposition to BisBAL NPs, Oxiselect Comet Assay was employed. Erythrocytes were incubated at 37°C and 5% of CO₂ overnight with 1–1000 μM of BisBAL NPs or 10% H₂O₂. Erythrocytes growing only in culture media were used as negative control (C⁻). After treatment, blood cells were analyzed by fluorescence microscopy at 485 nm (Thornwood, NY). The presence of a stela is indicative of DNA damage.