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Journal of Nanomaterials
Volume 2015, Article ID 367964, 9 pages
http://dx.doi.org/10.1155/2015/367964
Research Article

Electrospinning Preparation of Timosaponin B-II-Loaded PLLA Nanofibers and Their Antitumor Recurrence Activities In Vivo

1Department of General Surgery, 454th Hospital of PLA, Najing 210002, China
2Department of Pharmacy, 454th Hospital of PLA, Najing 210002, China
3Department of Pharmacy, Shanghai Changzheng Hospital, 415 Fengyang Road, Shanghai 200003, China
4Department of Pharmaceutical Science & Technology, College of Chemistry and Biology, Donghua University, 2999 North Renmin Road, Shanghai 201620, China

Received 30 June 2014; Revised 11 September 2014; Accepted 15 September 2014

Academic Editor: Fujuan Liu

Copyright © 2015 Zhonghua Huo et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Poly(L-lactic)-acid (PLLA) as a drug carrier and a water-soluble drug timosaponin B-II (TB-II) as a model drug were selected to prepare drug-loaded nanofibers by electrospinning. The average diameters of pure PLLA nanofibers and TB-II-loaded nanofibers were 212.5 ± 68.5, 219.7 ± 57.8, 232.8 ± 66.9, and 232.9 ± 97.7 nm, respectively. DSC and XRD results demonstrated that TB-II was well incorporated into the nanofibers in an amorphous state. FI-TR spectroscopy indicated that TB-II had good compatibility with PLLA. In vitro release studies showed that TB-II was rapidly released from the nanofibers within 6 h, followed by a gradual release for long time. In vivo biosafety test revealed no noticeable toxicity of these TB-II nanofibers. The TB-II released from the nanofibers had obvious inhibition effect against human hepatocellular carcinoma SMMC 7721 cells both in vivo and in vitro. It was confirmed that the TB-II-loaded nanofibers were a sustained delivery system which could effectively inhibit the tumor growth and recurrence after surgery.