Immobilization Techniques and Integrated Signal Enhancement for POC Nanocolor Microfluidic Devices

Figure 1

Classical REA setup and bioanalytical REA assay. (a) Schematic drawing of the classical 4-layer REA setup with two different resonance layer thicknesses. The colored squares above the metal cluster layers indicate the film color, which varies with resonance layer thickness. (b) Schematic drawing of a bioanalytical REA assay before (left) and after (right) binding of AuNP-analyte complexes to the capture protein layer. (Without top metal cluster REA materials exhibit the color of the base mirror layer, generally silver to gray.) The NP-analyte complex may comprise further mediator proteins, which are not depicted here. (c) REA color spectrum. By stepwise increase of the resonance layer thickness from 0 to 300 nm, a broad range of colors can be obtained.