Configuration Transitions of Free Circular DNA System Induced by Nicks

Figure 1

Nicking sites in pUC19 DNA were produced by two nicking endonucleases (a). The blue and green symbols represent Nb.BsrDI and Nt.BstNBI, respectively. Schematic of angle degrees on pUC19 DNA produced by two nicking endonucleases (b–d). Nicking angles of pUC19 DNA with (b) two, (c) four, and (d) six nicks.

(a)

(b)

(c)

(d)