Noninvasive 3D Structural Analysis of Arthropod by Synchrotron X-Ray Phase Contrast Tomography

Figure 1

Schematic diagram of the synchrotron X-ray in-line phase contrast imaging setup. A monochromatic X-ray with specific energy is selected by a monochromator from the synchrotron radiation X-ray beam. Fresnel diffraction occurs on the interfaces of different tissues when the beam passes through the sample. The phase contrast image is recorded by a detector when the detector and the sample are at an appropriate distance. During the tomographic scan, the specimen is rotated around the vertical axis at 180 degrees. In this experiment a mechanical shutter is used to avoid radiation damage of biological sample in the absence of image capture.