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Journal of Nanomaterials
Volume 2015, Article ID 607268, 15 pages
http://dx.doi.org/10.1155/2015/607268
Research Article

Functionalised Au Coated Iron Oxide Nanocomposites Based Reusable Immunosensor for AFB1 Detection

Amity Institute of Nanotechnology, Amity University, Uttar Pradesh, Noida 201301, India

Received 17 March 2015; Accepted 14 June 2015

Academic Editor: Tapan Desai

Copyright © 2015 Ruchika Chauhan and T. Basu. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

A reusable sandwiched electrochemical piezoelectric immunosensor has been developed for aflatoxin B1 (AFB1) detection using gold coated iron oxide core-shell (Au-Fe3O4) nanostructure. The monoclonal anti-aflatoxin antibody (aAFB1) was immobilized on self-assembled monolayer of 4-aminothiophenol on gold coated quartz crystal to fabricate immunoelectrode (BSA/aAFB1/4-ATP/Au). In addition, secondary rabbit-immunoglobulin antibodies (r-IgGs) functionalized with Au-Fe3O4 NPs via cysteamine (r-IgG-Cys-Au-Fe3O4) were allowed to interact with AFB1. Both competitive and noncompetitive strategies were employed and a competition between coated AFB1 and free AFB1 was carried out. The competitive mode shows higher linear range (0.05 to 5 ng mL−1) than the noncompetitive one (0.5 to 5 ng mL−1), high sensitivity 335.7 µA ng−1 mL cm−2, and LOD 0.07 ng mL−1. The fabricated immunosensor has been tested using cereal samples spiked with different concentrations of AFB1. The developed competitive immunoelectrode displays good reproducibility, and storage stability and regenerated with negligible loss in activity through removal of the r-IgG-Cys-Au-Fe3O4 conjugate using a strong external magnet.