Apoptosis Induction in Human Leukemia Cell Lines by Gold Nanoparticles Synthesized Using the Green Biosynthetic Approach

Table 2

Flowcytometric analysis of K562 cells after being treated with S-GNPs. The cells were stained with PI and incubated at 37°C for 12, 24, and 48 h.

Cell cycle phases	Cells %
	Control	Treated/12 h	Treated/24 h	Treated/48 h
G0/G1	54.33 ± 0.06	51.36 ± 0.45	50.56 ± 0.52	47.10 ± 0.68
G2/M	22.52 ± 0.76	27.14 ± 0.41	30.47 ± 0.35	33.83 ± 0.93
Synthesis	15.26 ± 0.06	18.22 ± 0.33	14.97 ± 0.12	13.11 ± 0.18
Sub-G0/G1	1.49 ± 0.23	3.39 ± 0.28	4.73 ± 0.20	6.69 ± 0.56

Values are expressed as mean ± SD of three different experiments. The data has been analyzed using post hoc comparison test-one way ANOVA, means compared with Tukey’s- test. (): significant () increased cells in G2/M phase in S-GNPs treated groups compared to untreated controls. (): significant () increased of apoptotic cells in sub-G0/G1 phase in S-GNPs treated groups compared to untreated controls.