Research Article
Iterative Cellular Screening System for Nanoparticle Safety Testing
Table 1
Sequences of the primers used in qPCR.
| | Gene name | Gene bank accession number (NCBI GenBank) | Location (bp) | Expected size of PCR amplicon (bp) | Sequence (5′-3′) |
| | GAPDH |
NM_002046.3 | 880–900 |
126 | F: AAGGTGGTGAAGCAGGCGTCG | | 1005–984 | R: AATGCCAGCCCCAGCGTCAAAG | | HPRT |
NM_000194.2 | 650–669 |
267 | F: AAGCTTGCTGGTGAAAAGGA | | 897–916 | R: AAGCAGATGCCCACACAACT |
| | For A549 cells | | | | | | IL-8 |
NM_000584.3 | 221–240 |
131 | F: CAGTTTTGCCAAGGAGTGCT | | 351–332 | R: AATTTCTGTGTTGGCGCAGT | | Hsp70 (HSPA1A) |
NM_005345.5 | 2049–2068 |
138 | F: GTGTAACCCCATCATCAGCG | | 2186–2166 | R: CAATCTTGGAAAGGCCCCTAA |
| | For NIH-3T3 cells | | | | | | IL-8 |
NM_011339.2 | 338–357 |
110 | F: GAATTTCCACCGGCAATGAA | | 447–428 | R: TCCCGAATTGGAAAGGGAAA | | Hsp70 (HSPA1A) |
NM_010478.2 | 2141–2161 |
104 | F: CATCGAGGAGGTGGATTAGAG | | 2244–2225 | R: ACCTTGACAGTAATCGGTGC |
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