Iterative Cellular Screening System for Nanoparticle Safety Testing

Table 3

Comparison of calculated IC₅₀ values for ZnO-NP of 2D cell culture and different 3D cell culture models. NIH-3T3 cells and A549 cells were attached on collagen scaffolds, encapsulated in hydrogel or aggregated in spheroids to grow in 3D. After exposure to ZnO-NP the viability of the cells was determined using either the MTT assay or the CTB assay. Values are means ± SD for ≥ 3. Data were already published by Sambale et al. 2015 [19].

	Viability assay	IC50 value μg/mL
		NIH-3T3 cells	A549 cells
2D	MTT	10 ± 3	58 ± 16
2D	CTB	15 ± 6	54 ± 12
3D scaffold	MTT	11 ± 2	75 ± 18
3D hydrogel	CTB	7 ± 3	328 ± 13
3D spheroids	CTB	9 ± 1	42 ± 13